Treatment of silymarin, a plant flavonoid, prevents ultraviolet light-induced immune suppression and oxidative stress in mouse skin.
Treatment of silymarin, a plant flavonoid, prevents ultraviolet light-induced immune suppression and oxidative stress in mouse skin.
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DOI：
10.3892/ijo.21.6.1213
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发表时间：
2002-12
影响因子：
5.2
通讯作者：
S. Katiyar
中科院分区：
文献类型：
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作者：
S. Katiyar
有充分证据表明，紫外线（UV）光诱导的免疫抑制和氧化应激在诱发皮肤癌中起重要作用。先前，我们已经证明水飞蓟素（一种来自水飞蓟的类黄酮）局部治疗小鼠皮肤可防止光致癌，但水飞蓟素在体内动物系统中预防光致癌的机制尚不清楚。为了明确预防机制，我们采用免疫染色、分析试验和ELISA方法，发现水飞蓟素（1 mg/cm2皮肤面积）局部治疗C3H/HeN小鼠可抑制UVB （90 mJ/cm2）诱导的接触致敏剂二硝基氟苯的接触超敏反应（CHS）。水飞蓟素预防uvb诱导的CHS抑制被发现与浸润性白细胞（特别是CD11b+细胞类型）和髓过氧化物酶活性的抑制有关（50-71%）。水飞蓟素处理也导致uvb诱导的免疫抑制细胞因子白介素-10产生细胞及其产量显著减少（58% -72%,p<0.001）。水飞蓟素局部处理也导致uva诱导的产生H2O2的细胞数量和诱导的表达一氧化氮合酶的细胞数量显著减少，H2O2 （58-65%, p<0.001）和一氧化氮（65-68%,p<0.001）的产生减少。总之，这些数据表明水飞蓟素预防uvb诱导的免疫抑制和氧化应激可能与预防小鼠光致癌有关。本研究数据还提示：1)水飞蓟素在皮肤癌高发人群中的i期临床试验；2)开发含水飞蓟素作为抗氧化剂（化学预防剂）的防晒霜或在护肤品中添加水飞蓟素。
It is well documented that ultraviolet (UV) light-induced immune suppression and oxidative stress play an important role in the induction of skin cancers. Earlier, we have shown that topical treatment of silymarin, a plant flavonoid from milk thistle (Silybum marianum L. Gaertn.), to mouse skin prevents photocarcinogenesis, but the preventive mechanism of photocarcinogenesis in vivo animal system by silymarin is not well defined and understood. To define the mechanism of prevention, we employed immunostaining, analytical assays and ELISA which revealed that topical treatment of silymarin (1 mg/cm2 skin area) to C3H/HeN mice inhibits UVB (90 mJ/cm2)-induced suppression of contact hypersensitivity (CHS) response to contact sensitizer dinitrofluorobenzene. Prevention of UVB-induced suppression of CHS by silymarin was found to be associated with the inhibition of infiltrating leukocytes, particularly CD11b+ cell type, and myeloperoxidase activity (50-71%). Silymarin treatment also resulted in significant reduction of UVB-induced immunosuppressive cytokine interleukin-10 producing cells and its production (58-72%, p<0.001). Topical treatment of silymarin also resulted in significant reduction of the number of UVB-induced H2O2 producing cells and inducible nitric oxide synthase expressing cells concomitant with decrease in H2O2 (58-65%, p<0.001) and nitric oxide (65-68%, p<0.001) production. Together, these data suggest that prevention of UVB-induced immuno-suppression and oxidative stress by silymarin may be associated with the prevention of photocarcinogenesis in mice. The data obtained from this study also suggest: i) phase-I clinical trial of silymarin in high skin cancer risk human population and ii) development of sunscreen containing silymarin as an antioxidant (chemopreventive agent) or silymarin can be supplemented in skin care products.