PRIMARY STRUCTURE OF LEADER RNA AND NUCLEOPROTEIN GENES OF THE RABIES GENOME - SEGMENTED HOMOLOGY WITH VSV
PRIMARY STRUCTURE OF LEADER RNA AND NUCLEOPROTEIN GENES OF THE RABIES GENOME - SEGMENTED HOMOLOGY WITH VSV
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DOI：
10.1093/nar/14.6.2671
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发表时间：
1986-03-25
影响因子：
14.9
通讯作者：
KEITH, G
中科院分区：
文献类型：
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作者：
TORDO, N;POCH, O;KEITH, G
我们测定了狂犬病病毒（PV株）基因组3 '端的核苷酸序列。这项工作是旨在建立完整一级结构的项目的第一步。从RNA基因组的3 '核苷酸序列，构建与3'末端互补的十八核苷酸，并用于引发cDNA合成。两个重叠的重组cDNA克隆杂交的核蛋白mRNA（NmRNA）的分离和测序。狂犬病基因组的前1500个核苷酸覆盖两个转录克隆单位：前导RNA和NmRNA，其通过SI核酸酶保护实验显示在残基59周围起始。狂犬病PV和CVS株之间的比较高达残基180表明在前导区的快速进化。对狂犬病毒和水泡性口炎病毒（VSV）两种弹状病毒3 '区序列的比较研究表明，它们之间存在分段同源性。在N蛋白中观察到可能参与与RNA基因组相互作用的高度保守氨基酸的延伸，尽管在其余序列中存在很大分歧。此外，转录起始和终止信号之间的高度同源性反映了这两种非节段负链RNA病毒中相似转录机制的保守性。
We have determined the nucleotide sequence of the 3'region of the rabies genome (PV strain). This work is a first step in a project aimed at establishing the complete primary structure. From the 3'nucleotide sequence of the RNA genome, an octadecanucleotide complementary to the 3'extremity was constructed and used to prime cDNA synthesis. Two overlapping recombinant cDNA clones hybridizing with the nucleoprotein mRNA (NmRNA) were isolated and sequenced. The 1500 first nucleotides of the rabies genome cover two transcriptlonal units: the leader RNA and the NmRNA which was shown to be initiated around residue 59 by SI nuclease protection experiments. Comparison between rabies PV and CVS strains up to residue 180 suggests a rapid evolution in the leader region. Studies of the sequence relationships between the 3'regions of two Rhabdoviruses, rabies virus and Vesicular Stomatitis Viruus (VSV), demonstrate that there is a segmented homology. Stretches of highly conserved amino acids possibly involved in the interaction with the RNA genome were observed in the N protein, despite a wide divergence in the remaining sequence. In addition, the high homology between the transcription start and stop signals reflects the conservation of a similar transcriptional mechanism in these two non segmented negative strand RNA viruses.