DIVERSITY OF CHLAMYDIA-TRACHOMATIS MAJOR OUTER-MEMBRANE PROTEIN GENES
DIVERSITY OF CHLAMYDIA-TRACHOMATIS MAJOR OUTER-MEMBRANE PROTEIN GENES
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DOI：
10.1128/jb.169.9.3879-3885.1987
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发表时间：
1987-09-01
影响因子：
3.2
通讯作者：
URDEA, MS
中科院分区：
文献类型：
--
作者：
STEPHENS, RS;SANCHEZPESCADOR, R;URDEA, MS
利用BamHI片段构建了沙眼衣原体血清型B和C的基因组DNA文库，并对含有各血清型主要外膜蛋白（omp 1）基因的重组子进行鉴定和测序。这些基因序列与来自血清型L2的基因之间的比较表明，血清型L2和B之间的碱基对差异小于L2和C之间的碱基对差异;这一发现与这些血清型之间的血清学和抗原关系一致。血清型L2和B的主要外膜蛋白（MOMP）的翻译氨基酸序列含有相同数量的氨基酸，而血清型C MOMP含有3个额外的氨基酸。衣原体MOMP的抗原多样性反映在四个序列可变区是推定的类型特异性抗原决定簇的候选者。研究了不同种C.观察到沙眼血清型对于密切相关的血清型是成簇的核苷酸取代，而对于远距离相关的血清型是插入或缺失。
Genomic DNA libraries were constructed for Chlamydia trachomatis serovars B and C by using BamHI fragments, and recombinants that contained the major outer membrane protein (omp1) gene for each serovar were identified and sequenced. Comparisons between these gene squences and the gene from serovar L2 demonstrated fewer base pair differences between serovars L2 and B than between L2 and C; this finding is consistent with the serologic and antigenic relationships among these serovars. The translated amino acid sequence for the major outer membrane proteins (MOMPs) contained the same number of amino acids for serovars L2 and B, whereas the serovar C MOMP contained three additional amino acids. The antigenic diversity of the chlamydial MOMP was reflected in four sequence-variable domains were candidates for the putative type-specific antigenic determinant. The molecular basis of omp1 gene diversity among C. trachomatis serovars was observed to be clustered nucleotide substitutions for closely related serovars and insertions or deletions for distantly related serovars.