Rhizobium pseudoryzae sp. nov., isolated from the rhizosphere of rice.
Rhizobium pseudoryzae sp. nov., isolated from the rhizosphere of rice.
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DOI：
10.1099/ijs.0.026146-0
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发表时间：
2011-10
影响因子：
2.8
通讯作者：
Xiaoxia Zhang;Lei Sun;Xiaotong Ma;X. Sui;Ruibo Jiang
中科院分区：
文献类型：
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作者：
Xiaoxia Zhang;Lei Sun;Xiaotong Ma;X. Sui;Ruibo Jiang
从新鲜水稻根部分离到一株革兰氏阴性好氧杆状细菌，命名为J3-A127（T）。细胞无运动性，未检测到鞭毛。16 S rRNA基因序列分析表明，该菌株与根瘤菌属Rhizobium Alt 505（T）的亲缘关系最近（96.4%）。菌株J3-A127（T）的gyrB、atpD、recA和gln Ⅱ基因序列与根瘤菌属已知种的模式菌株之间的低水平（<90%）的16 S rRNA基因序列相似性也表明它代表一个单独的种。生长的温度范围为10-40 °C（最佳约28 °C），pH范围为6.0-11.0（最佳pH 7.0-8.0）。菌株J3-A127（T）耐受高达5.0%（w/v）的NaCl浓度。该菌株为过氧化氢酶和氧化酶阳性。主要的细胞脂肪酸是特征8的总和（C（18：1）ω 7 c和/或C（18：1）ω6; 46.7%）。菌株J3-A127（T）的DNA G+C含量为59.5mol%。菌株J3-A127（T）在4种豆科植物上均未结瘤，PCR检测也未检测到nifH基因和nifD基因。根据生理生化特性和基因型数据，菌株J3-A127（T）被认为是根瘤菌属的一个新种，并建议将其命名为Rhizobium pseudoryrhizoma sp. nov.。模式菌株为J3-A127（T）（= ACCC 10380（T）= KCTC 23294（T））。
A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain J3-A127(T), was isolated from the roots of fresh rice plants (Oryza sativa). Cells were non-motile and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus Rhizobium, with closest similarity to Rhizobium oryzae Alt 505(T) (96.4 %). The low levels of 16S rRNA gene sequence similarity (<90 %) found between the gyrB, atpD, recA and glnII gene sequences of strain J3-A127(T) and the type strains of recognized species of the genus Rhizobium also indicated that it represented a separate species. The temperature range for growth was 10-40 °C (optimum around 28 °C) and the pH range was 6.0-11.0 (optimum pH 7.0-8.0). Strain J3-A127(T) tolerated NaCl concentrations up to 5.0 % (w/v). The strain was catalase- and oxidase-positive. The main cellular fatty acids were summed feature 8 (C(18 : 1)ω7c and/or C(18 : 1)ω6; 46.7 %). The DNA G+C content of strain J3-A127(T) was 59.5 mol%. Strain J3-A127(T) did not form any nodules on four different legumes and the nodD and nifH genes were not detected by PCR. According to physiological and biochemical characteristics and genotypic data, strain J3-A127(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium pseudoryzae sp. nov. is proposed. The type strain is J3-A127(T) ( = ACCC 10380(T) = KCTC 23294(T)).