Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex.
Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex.
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DOI：
10.7554/elife.60810
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发表时间：
2021-07-12
期刊：
影响因子：
7.7
通讯作者：
Butt SJ
中科院分区：
文献类型：
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作者：
Ghezzi F;Marques-Smith A;Anastasiades PG;Lyngholm D;Vagnoni C;Rowett A;Parameswaran G;Hoerder-Suabedissen A;Nakagawa Y;Molnar Z;Butt SJ
亚板神经元（spn）被认为在新皮层的新生感觉加工中起作用。为了更好地了解这一群体的异质性与突发性功能之间的关系，我们研究了出生后第一周在须状体感皮层（S1BF）中Lpar1-EGFP spn的突触连接。这些spn包括两种形态亚型：具有局部轴突的梭状spn和轴突延伸到边缘区的锥体spn。前者接受跨层突触输入，直到须状突起出现，这个时间点与显著的细胞死亡一致。相反，锥体spn在早期接受来自底板的局部输入，但随后在较晚的时间窗口内从上覆皮层获取输入。丘脑传入事件的电和光遗传学联合激活发现Lpar1-EGFP spn接受稀疏的丘脑神经支配。这些数据揭示了出生后网络的组成部分，该网络解释了稀疏的丘脑输入，以指导S1BF出现的柱状结构。
Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF.