JUVENILE-HORMONE METABOLISM DURING THE 4TH STADIUM AND PUPAL STAGE OF THE SOUTHERN HOUSE MOSQUITO, CULEX-QUINQUEFASCIATUS SAY
JUVENILE-HORMONE METABOLISM DURING THE 4TH STADIUM AND PUPAL STAGE OF THE SOUTHERN HOUSE MOSQUITO, CULEX-QUINQUEFASCIATUS SAY
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DOI：
10.1016/0022-1910(95)00037-u
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发表时间：
1995-10-01
影响因子：
2.2
通讯作者：
ROE, RM
中科院分区：
文献类型：
--
作者：
LASSITER, MT;APPERSON, CS;ROE, RM
致倦库蚊（Culex quinquefasciatus）保幼激素（JH）酯酶活性在4龄时变化不显著，但JH环氧化物水解酶活性在36 h龄时达到峰值，分别为JH酯酶活性的5.7倍和3.0倍，在整个幼虫发育过程中和0 h龄蛹中JH环氧化物水解酶活性均大于JH酯酶活性。这两种活动下降，相当于在pupelies超过8小时的老。1-醋酸萘（NA）酯酶活性在第四龄期间保持恒定，在16-24 h龄的蛹中达到峰值。在四龄和蛹中发现了不同等电形式的JH酯酶和环氧化物水解酶。亚细胞分布研究表明，大部分JH酯酶和JH环氧化物水解酶是膜结合的，O，O-二异丙基氟磷酸酯酶的I-50是1-NA酯酶的3000倍，但在8 h龄以上的蛹中，两种酶的I-50值大致相等。但不能延长蛹期的持续时间。Methoprene还消除了在最后一个体育场期间发现的JH环氧化物水解酶活性的正常峰，增加了1-NA酯酶活性，但对JH酯酶活性没有影响。甲氧普烯处理对蛹的JH环氧化物水解酶和JH酯酶活性没有影响，但抑制了蛹的1-NA酯酶活性，消除了蛹的1-NA酯酶峰。
Juvenile hormone (JH) esterase activity did not change significantly during the fourth stadium in Culex quinquefasciatus, but JH epoxide hydrolase activity peaked in 36-h-old fourth instars and pharate pupae at levels 5.7- and 3.0-times that of JH esterase, respectively, JH epoxide hydrolase activity was greater than the JH esterase activity throughout larval development and in 0-h-old pupae. Both activities declined and were equivalent in pupae more than 8-h-old. 1-Naphthyl acetate (NA) esterase activity remained constant during the fourth stadium and peaked in pupae 16-24-h-old, Different isoelectric forms of JH esterase and epoxide hydrolase were found in fourth instars and pharate pupae, Subcellular distribution studies indicated that the majority of JH esterase and JH epoxide hydrolase were membrane bound, Inhibition of larval JH esterase with O,O-diisopropyl phosphorofluoridate revealed an I-50 3000-times that of 1-NA esterase but both enzymes had approximately equivalent I-50 values in pupae that were more than 8-h-old, Methoprene treatment of fourth instars significantly extended the duration of the fourth stadium, but did not extend the duration of the pupal stage. Methoprene also eliminated the normal peaks in JH epoxide hydrolase activity found during the last stadium, increased the 1-NA esterase activity, but had no effect on JH esterase activity. Methoprene treatment of fourth instars had no effect on the pupal JH epoxide hydrolase and JH esterase activity but depressed the overall 1-NA esterase activity eliminating the pupal 1-NA esterase peak.