Gene regulatory control in the sea urchin aboral ectoderm: Spatial initiation, signaling inputs, and cell fate lockdown
Gene regulatory control in the sea urchin aboral ectoderm: Spatial initiation, signaling inputs, and cell fate lockdown
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DOI：
10.1016/j.ydbio.2012.11.013
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发表时间：
2013-02-01
影响因子：
2.7
通讯作者：
Davidson, Eric H.
中科院分区：
文献类型：
--
作者：
de-Leon, Smadar Ben-Tabou;Su, Yi-Hsien;Davidson, Eric H.
近年来，海胆胚胎口-口外胚层发育的调控得到了广泛的研究。口-口极性最初施加于氧化还原梯度的下游，这种氧化还原梯度是由母体线粒体的不对称分布引起的。两条tgf - β信号通路。然后分别利用Nodal和BMP来产生口服和分娩调节状态。然而，对流产外胚层规格调节的因果理解一直缺乏。在这项工作中，通过结合详细的调控基因表达研究，扰动和顺式调控分析揭示了体外外胚层调控状态规范的控制。我们的分析揭示了一个动态系统，不同的因素在不同的发展时期占主导地位。我们发现流产基因的初始激活直接取决于氧化还原敏感转录因子，缺氧诱导因子1 α (HIF-1 α)。两种BMP配体BMP2/4和BMP5/8显著增强了体外调控基因的转录。最终，编码反馈线路锁定了体外胚层的调节状态。我们的研究阐明了不同的调控机制，这些机制依次主导着流产调控状态的空间定位。(C) 2012爱思唯尔公司版权所有。
The regulation of oral-aboral ectoderm specification in the sea urchin embryo has been extensively studied in recent years. The oral-aboral polarity is initially imposed downstream of a redox gradient induced by asymmetric maternal distribution of mitochondria. Two TGF-beta signaling pathways. Nodal and BMP, are then respectively utilized in the generation of oral and aboral regulatory states. However, a causal understanding of the regulation of aboral ectoderm specification has been lacking. In this work control of aboral ectoderm regulatory state specification was revealed by combining detailed regulatory gene expression studies, perturbation and cis-regulatory analyses. Our analysis illuminates a dynamic system where different factors dominate at different developmental times. We found that the initial activation of aboral genes depends directly on the redox sensitive transcription factor, hypoxia inducible factor 1 alpha (HIF-1 alpha). Two BMP ligands, BMP2/4 and BMP5/8, then significantly enhance aboral regulatory gene transcription. Ultimately, encoded feedback wiring lockdown the aboral ectoderm regulatory state. Our study elucidates the different regulatory mechanisms that sequentially dominate the spatial localization of aboral regulatory states. (C) 2012 Elsevier Inc. All rights reserved.