Physical Mapping of the Genome of Rhodobacter Capsulatus
Physical Mapping of the Genome of Rhodobacter Capsulatus
复制标题
荚膜红杆菌基因组的物理作图
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
S. Brenner
中科院分区：
文献类型：
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作者：
J. Williams;B. Marrs;S. Brenner
在光合细菌中，荚膜红杆菌（Rhodobacter capsulatus）的遗传特性已经得到了很好的研究（参见Scolnik和Marrs， 1987）。通过各种精细结构遗传作图技术，先前已经确定，该生物中参与光合机构合成的大多数基因都聚集在基因组的一个小区域（Yen和Marrs， 1976），并且该区域已被克隆（Marrs, 1981； Taylor等人，1983）)并对部分基因进行了测序（Youvan等人，1984）。通过R质粒介导的偶联作图产生了染色体的环状连锁图谱（Willison等，1985）。对荚膜菇染色体整体结构的详细研究有助于了解荚膜菇基因的全局调控，而对荚膜菇基因组各部分克隆的可用性将有助于其他基因的鉴定和测序。为了达到这些目的，我们正在确定荚膜菌染色体的物理图谱。
Among the photosynthetic bacteria, Rhodobacter capsulatus has been particularly well characterized genetically (reviewed in Scolnik and Marrs, 1987). By a variety of fine structure genetic mapping techniques, it has been previously determined that most of the genes involved in the synthesis of the photosynthetic apparatus in this organism are clustered in a small region of the genome (Yen and Marrs, 1976), and that region has been cloned (Marrs, 1981; Taylor, et al., 1983)) and some of the genes sequenced (Youvan, et al., 1984). Mapping by R plasmid-mediated conjugation has produced a circular linkage map of the chromosome (Willison, et al., 1985). A detailed study of the overall organization of the chromosome of R. capsulatus should assist in the understanding of the global regulation of its genes, and the availability in cloned form of all parts of the genome will facilitate the identification and sequencing of additional genes. Towards these ends we are determining a physical map of the chromosome of R. capsulatus.