Elevated tyrosine hydroxylase in the locus coeruleus of suicide victims.
Elevated tyrosine hydroxylase in the locus coeruleus of suicide victims.
复制标题
自杀受害者蓝斑中酪氨酸羟化酶升高。
DOI：
10.1046/j.1471-4159.1994.62020680.x
复制
发表时间：
1994
影响因子：
4.7
通讯作者：
Haycock,JW
中科院分区：
文献类型：
--
作者：
Ordway,GA;Smith,KS;Haycock,JW
通过定量印迹免疫标记细胞核尾部的低温恒温切片，测定了9对无抗抑郁药自杀患者和年龄匹配的猝死对照病例的蓝斑中酪氨酸羟化酶蛋白的含量。在9个年龄匹配的配对中，自杀受害者的样本中酪氨酸羟化酶的浓度更高，数值范围为匹配对照值的108 - 172%（x = 136%）。相比之下，在同一组样品中，神经元特异性烯醇化酶蛋白的浓度没有差异。同样，两组之间的蓝斑切片中含有神经黑色素的细胞数量（与用于印迹免疫标记分析的切片相邻）没有差异。这些数据表明，自杀受害者的蓝斑神经元含有高于正常浓度的酪氨酸羟化酶，从而提高了蓝斑中酪氨酸羟化酶的表达可能与自杀的病理生理学相关的可能性。
The amounts of tyrosine hydroxylase protein in locus coeruleus from nine pairs of antidepressant‐free suicide victims and age‐matched, sudden‐death control cases were determined by quantitative blot immunolabeling of cryostat‐cut sections from the caudal portion of the nucleus. In each of the nine age‐matched pairs, the concentration of tyrosine hydroxylase was greater in the sample from the suicide victim, with values ranging from 108 to 172% of the matched control value (\‐x = 136%). By contrast, there were no differences in the concentrations of neuron‐specific enolase protein in the same set of samples. Similarly, the number of neuromelanin‐containing cells, counted in sections of locus coeruleus adjacent to those taken for blot immunolabeling analyses, did not differ between the two groups. These data indicate that locus coeruleus neurons from suicide victims contain higher than normal concentrations of tyrosine hydroxylase, thus raising the possibility that the expression of tyrosine hydroxylase in locus coeruleus may be relevant in the pathophysiology of suicide.