Solubilization and characterization of the leukotriene C4 synthetase of rat basophil leukemia cells: a novel, particulate glutathione S-transferase.
Solubilization and characterization of the leukotriene C4 synthetase of rat basophil leukemia cells: a novel, particulate glutathione S-transferase.
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大鼠嗜碱性粒细胞白血病细胞白三烯 C4 合成酶的溶解和表征：一种新型颗粒谷胱甘肽 S-转移酶。
DOI：
10.1016/0003-9861(84)90426-0
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发表时间：
1984
影响因子：
3.9
通讯作者：
D. R. Morton
中科院分区：
文献类型：
--
作者：
M. K. Bach;J. R. Brashler;D. R. Morton
在谷胱甘肽存在下，大鼠嗜碱性粒细胞白血病细胞匀浆有效地催化白三烯A4转化为白三烯C4和D4的混合物。用分光光度法测定，这些匀浆还催化卤代硝基苯与谷胱甘肽的加合物的形成。虽然所有经典的谷胱甘肽转移酶活性都存在于匀浆的可溶性部分，但硫醚白三烯的生成活性存在于颗粒部分。这种“白三烯C合成酶”活性是通过非离子洗涤剂Triton X-100从粗制的高速颗粒组分中溶解出来的。在谷胱甘肽存在的情况下，溶解的酶不能将2，4-二硝基氯苯转化为有色产物。它也不会与3，4-二氯硝基苯反应。另一方面，在最佳条件下，该酶制剂能够在至少10min的线性反应中产生约0.1nmoL的白三烯C mg蛋白−1min−1。底物特异性的这种解离以及抑制谱的差异，将大鼠嗜碱性粒细胞匀浆中的酶活性与已在大鼠肝匀浆中描述的微粒体谷胱甘肽转移酶区分开来，表明这种“白三烯C合成酶”是一种新的独特的酶。
Rat basophil leukemia cell homogenates effectively catalyze the conversion of leukotriene A4to a mixture of leukotrienes C4and D4in the presence of glutathione. These homogenates also catalyze the formation of adducts of halogenated nitrobenzene with glutathione, as determined spectrophotometrically. While all the classical glutathioneS-transferase activity resides in the soluble fraction of the homogenates, the thiol ether leukotriene-generating activity is found in the particulate fraction. This “leukotriene C synthetase” activity has been solubilized from a crude high-speed particulate fraction by means of the nonionic detergent, Triton X-100. The solubilized enzyme is incapable of converting 2,4-dinitrochlorobenzene to a colored product in the presence of glutathione. Nor will it react with 3,4-dichloronitrobenzene. On the other hand, under optimal conditions, this enzyme preparation is capable of generating about 0.1 nmol leukotriene C mg protein−1min−1in a reaction which continues in linear fashion for at least 10 min. This dissociation in substrate specificity, as well as differences in the inhibition profile, distinguish the enzyme activity in the particulate fraction from rat basophil leukemia cell homogenates from the microsomal glutathioneS-transferase which has been described in rat liver homogenates, suggesting that this “leukotriene C synthetase” is a new and unique enzyme.
DOI：
--
发表时间：
1982
期刊：
European journal of biochemistry / FEBS
影响因子：
--
作者：
Morgenstern,R;Guthenberg,C;Depierre,JW
通讯作者：
Depierre,JW