Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization
Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization
复制标题
DOI：
10.1073/pnas.1701484114
复制
发表时间：
2017-05-16
影响因子：
11.1
通讯作者：
McDermott, Ann E.
中科院分区：
文献类型：
--
作者：
Sergeyev, Ivan V.;Itin, Boris;McDermott, Ann E.
提出了一种提高固体核磁共振研究中动态核极化效率的实验方法。该方法通过侧链和C α共振的顺序相关性使分配更简单，更快速，更可靠。该方法特别适合于复杂的生物分子和系统具有显着的化学位移简并性。它的目的是克服光谱拥挤和线加宽，发生由于样品冷冻在低温下所需的DNP。非均匀采样（NUS）被纳入，以实现时间效率的多维数据的收集。此外，快速（25 kHz）魔角旋转（MAS）提供了最佳的灵敏度和分辨率。中收集的数据
An experimental strategy has been developed to increase the efficiency of dynamic nuclear polarization (DNP) in solid-state NMR studies. The method makes assignments simpler, faster, and more reliable via sequential correlations of both side-chain and C alpha resonances. The approach is particularly suited to complex biomolecules and systems with significant chemical-shift degeneracy. It was designed to overcome the spectral congestion and line broadening that occur due to sample freezing at the cryogenic temperatures required for DNP. Nonuniform sampling (NUS) is incorporated to achieve time-efficient collection of multidimensional data. Additionally, fast (25 kHz) magic-angle spinning (MAS) provides optimal sensitivity and resolution. Data collected in