Biological Characterization of Marssonina coronaria Associated with Apple Blotch Disease.
Biological Characterization of Marssonina coronaria Associated with Apple Blotch Disease.
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DOI：
10.5941/myco.2011.39.3.200
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发表时间：
2011-09
期刊：
影响因子：
1.9
通讯作者：
Jung HY
中科院分区：
文献类型：
--
作者：
Lee DH;Back CG;Win NK;Choi KH;Kim KM;Kang IK;Choi C;Yoon TM;Uhm JY;Jung HY
与苹果斑点病相关的Marssonina coronaria导致严重的过早落叶，并且在韩国广泛分布。从2005~2007年在韩国庆北省的果园中采集到13个分离物。所有菌株的内转录间隔区和28 SrDNA部分序列的相似性分别为99.6%和99.2%。这些分离物与中国分离物的亲缘关系密切。所选菌株的致病性没有差异。该菌在马铃薯蛋白胨葡萄糖琼脂（PPDA）上生长的最适条件为20℃、pH 6。蛋白胨和甘露糖分别是最佳的氮源和碳源。真菌在PPDA上的生长优于普通马铃薯葡萄糖琼脂。本研究为综合疾病管理方案提供了有价值的信息，并促进了M. coronaria。
Marssonina coronaria associated with apple blotch disease causes severe premature defoliation, and is widely distributed in Korea. Thirteen isolates were collected from orchards located in Gyeongbuk Province from 2005~2007. All isolates displayed over 99.6% and 99.2% sequence similarity to each other in internal transcribed spacer regions and partial sequences of 28S rDNA, respectively. The isolates were phylogenetically closely related to Chinese isolates. Selected isolates did not differ in their pathogenicity. The optimum conditions for fungal growth were 20℃ and pH 6 on peptone potato dextrose agar (PPDA). Peptone and mannose were the best nitrogen and carbon source, respectively. Fungal growth was better on PPDA than on common potato dextrose agar. This study provides valuable information for integrated disease management program and facilitates the routine culturing of M. coronaria.