Foetal exposure to phthalate esters and anogenital distance in male newborns
Foetal exposure to phthalate esters and anogenital distance in male newborns
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DOI：
10.1111/j.1365-2605.2011.01190.x
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发表时间：
2012-06-01
影响因子：
--
通讯作者：
Shiraishi, H.
中科院分区：
文献类型：
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作者：
Suzuki, Y.;Yoshinaga, J.;Shiraishi, H.
邻苯二甲酸酯是常用的增塑剂，具有抗雄激素活性，在实验动物中引起雄性生殖畸形。然而，产前接触邻苯二甲酸酯对人类的影响尚未得到广泛研究。本研究的目的是检查产前暴露于邻苯二甲酸酯和肛门生殖器距离（AGD）作为人类男性新生儿生殖终点之间的关系。在获得111名日本孕妇的知情同意后，采集了她们的尿样。采用高效液相色谱-串联质谱法测定尿中7种邻苯二甲酸酯代谢物。尿异黄酮浓度被测量为可能的协变量，因为它们的雌激素性和日本人的高暴露水平有可能影响男性生殖器发育。研究人员测量了男性新生儿的出生结果和肛门中心到外生殖器的距离AGD。在多元回归模型中，当控制潜在协变量时，对数转换的邻苯二甲酸单-2-乙基己基酯浓度（比重校正）在解释肛门生殖指数（AGI）时呈负显著性，而母亲吸烟状况呈显著性。在所有模型中，尿异黄酮对AGI均无显著影响。我们的研究结果表明，产前接触邻苯二甲酸二（2-乙基己基）会影响人类男性的生殖发育。
Phthalate esters, commonly used as plasticizers, show anti-androgenic activity and cause male reproductive malformation in experimental animals. However, the effects of prenatal exposure to phthalate esters in humans have not been extensively studied. The purpose of this study was to examine the relationship between prenatal exposure to phthalate esters and the anogenital distance (AGD) as a reproductive endpoint in human male newborns. Spot urine samples were collected from 111 Japanese pregnant women after obtaining their informed consent. Seven urinary phthalate ester metabolites were determined by high performance liquid chromatographytandem mass spectrometry. Urinary isoflavones concentrations were measured as possible covariates because their oestrogenicities and high exposure levels among Japanese have the potential to affect male genital development. Birth outcomes and AGD, the distance from the centre of the anus to external genitalia, were measured for their male newborns. In a multiple regression model, the log-transformed mono-2-ethylhexyl phthalate concentration (specific gravity-corrected) was negatively significant, and maternal smoking status was positively significant, in explaining anogenital index (AGI) when potential covariates were controlled for. Urinary isoflavones did not significantly contribute to AGI in any models. Our results suggest that prenatal exposure to di(2-ethylhexyl) phthalate affects reproductive development in human males.