Wolbachia Effects on Host Fitness and the Influence of Male Aging on Cytoplasmic Incompatibility in Aedes polynesiensis (Diptera: Culicidae)
Wolbachia Effects on Host Fitness and the Influence of Male Aging on Cytoplasmic Incompatibility in Aedes polynesiensis (Diptera: Culicidae)
复制标题
DOI：
10.1603/me10202
复制
发表时间：
2011-09-01
影响因子：
2.1
通讯作者：
Dobson, S. L.
中科院分区：
文献类型：
--
作者：
Brelsfoard, C. L.;Dobson, S. L.
内共生细菌Wolbachia通过诱导一种被称为细胞质不亲和性(CI)的不育形式来操纵宿主繁殖，促进感染入侵自然宿主种群。CI因用于控制病媒昆虫的应用策略而受到关注。因此，要了解自然发生的沃尔巴克氏菌入侵并评估潜在的应用策略，重要的是了解沃尔巴克氏菌与宿主的相互作用，包括对适合度和CI水平的影响。在这项研究中，我们检测了沃尔巴克氏杆菌存活、发育时间、性别比、寿命、繁殖力和卵孵化的影响，该标记是南太平洋班克罗夫氏原生伊蚊的主要媒介。在这项研究中，我们比较了自然和人工感染沃尔巴克氏菌的波氏拟青霉菌株和其他非共生菌菌株(去除沃尔巴克氏菌以产生未感染的菌株)。与非共生菌株相比，人工感染菌株的幼虫死亡率增加，成虫寿命降低。观察到，与非共生菌株相比，自然感染菌株的幼虫死亡率、幼虫死亡率、成虫寿命延长，成虫体型更大。4周龄的人工感染雄鼠能够诱发高发病率的CI，与年轻雄鼠相似。我们讨论了与沃尔巴克氏菌的自然传播有关的结果，以及基于沃尔巴克氏菌的应用策略来修改多尼苏达线虫种群。
The endosymbiotic bacteria Wolbachia manipulate host reproduction by inducing a form of sterility known as cytoplasmic incompatibility (CI), promoting the invasion of infection into natural host populations. CI has received attention for use in applied strategies to control insect vectors of disease. Thus, to understand both naturally occurring Wolbachia invasions and evaluate potential applied strategies, it is important to understand Wolbachia interactions with its host, including impacts on fitness and the CI level. In this study, we examined for an effect of Wolbachia cm survivorship, developmental time, sex ratio, longevity, fecundity, and egg hatch of Aedes polynesiensis Marks, which is the primary vector of Wuchereria bancrofti in the South Pacific. In this study, we have compared strains of A. polynesiensis that are naturally and artificially infected with Wolbachia and additional strains that are aposymbiotic (Wolbachia removed to generate an uninfected strain). Artificially infected strains were observed to have increased larval mortality and decreased adult longevity when compared with aposymbiotic strains. Naturally infected strains were observed to have decreased larval mortality, pupal mortality, increased adult longevity, and a larger adult size when compared with aposymbiotic strains. Artificially infected males that were 4 wk old were able to induce high rates of CI, similar to young males. We discuss the results in relation to the natural spread of Wolbachia and Wolbachia-based applied strategies to modify A. polynesiensis populations.