Cryptosporidium hominis n. sp (Apicomplexa: Cryptosporidiidae) from Homo sapiens
Cryptosporidium hominis n. sp (Apicomplexa: Cryptosporidiidae) from Homo sapiens
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DOI：
10.1111/j.1550-7408.2002.tb00224.x
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发表时间：
2002-11-01
影响因子：
2.2
通讯作者：
Xiao, LH
中科院分区：
文献类型：
--
作者：
Morgan-Ryan, UM;Fall, A;Xiao, LH
本文描述了一种新种隐孢子虫的卵囊结构和感染性。卵囊在结构上与微小隐孢子虫的卵囊难以区分。新种卵囊长宽比1.0-1.09(平均1.07)(n=100)，长宽比1.0-1.09(平均1.07)(n=100)。卵囊对ARC Swiss小鼠、裸鼠、Wistar大鼠幼鼠、幼犬、小猫或小牛不具感染性，但对新生灵知菌猪具有感染性。在生猪模型中的致病性研究表明，微小弧菌与这一新描述的人类物种在寄生虫相关病变的分布(P=0.005.0 5~P=0.0 2)和感染强度(P=0.0 4)方面存在显著差异。体外培养研究也揭示了这两个物种之间的生长差异。对多个非连锁位点的分析，包括对整个基因组的初步序列扫描，表明该物种与微小弧菌不同，也表明缺乏重组，为其物种地位提供了进一步的支持。根据生物学和分子生物学资料，该隐孢子虫被认为是人隐孢子虫的一个新种。
The structure and infectivity of the oocysts of a new species of Cryptosporidium from the feces of humans are described. Oocysts are structurally indistinguishable from those of Cryptosporidium parvum. Oocysts of the new species are passed fully sporulated, lack sporocysts, and measure 4.4-5.4 mum (mean = 4.86) X 4.4-5.9 mum (mean = 5.2 mum) with a length to width ratio 1.0-1.09 (mean 1.07) (n = 100). Oocysts were not infectious for ARC Swiss mice, nude mice, Wistar rat pups, puppies, kittens or calves, but were infectious to neonatal gnotobiotic pigs. Pathogenicity studies in the gnotobiotic pig model revealed significant differences in parasite-associated lesion distribution (P = 0.005 to P = 0.02) and intensity of infection (P = 0.04) between C. parvum and this newly described species from humans. In vitro cultivation studies have also revealed growth differences between the two species. Multi-locus analysis of numerous unlinked loci, including a preliminary sequence scan of the entire genome demonstrated this species to be distinct from C. parvum and also demonstrated a lack of recombination, providing further support for its species status. Based on biological and molecular data, this Cryptosporidium infecting the intestine of humans is proposed to be a new species Cryptosporidium hominis n. sp.