CALCIUM REGULATION OF CELL-CELL CONTACT AND DIFFERENTIATION OF EPIDERMAL-CELLS IN CULTURE - AN ULTRASTRUCTURAL-STUDY
CALCIUM REGULATION OF CELL-CELL CONTACT AND DIFFERENTIATION OF EPIDERMAL-CELLS IN CULTURE - AN ULTRASTRUCTURAL-STUDY
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DOI：
10.1016/0014-4827(83)90115-5
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发表时间：
1983-01-01
影响因子：
3.7
通讯作者：
HOLBROOK, KA
中科院分区：
文献类型：
--
作者：
HENNINGS, H;HOLBROOK, KA
通过透射和扫描电子显微镜研究了钙对培养的角质形成细胞生长的调节作用。在标准培养条件（1.2- 1.8mM Ca）下，细胞通过桥粒连接并分层为4-6个细胞层。体外表皮成熟的许多方面与体内过程类似，包括透明角质颗粒的形成、细胞核的丢失、皮质包膜的形成和含有角蛋白丝的皮质细胞的脱落。当培养基Ca浓度降低到0.02- 0.1M时，角质形成细胞的生长模式发生显著变化。细胞生长为单层，没有桥粒连接，并迅速增殖，大量非皮质细胞脱落到培养基中。大束角蛋白丝集中在核周细胞质中。细胞外Ca升高至1.2 mM诱导低Ca角质形成细胞分层、角质化和角化，其方式类似于在标准Ca培养基中铺板时所见。钙诱导的最早的超微结构变化是相邻细胞间桥粒的不对称形成。加入Ca后5 min观察到桥粒斑块和张力原丝; 1-2 h内形成对称的桥粒。该系统为研究桥粒组装和拆卸的调控提供了一个有用的模型。
Ca modulation of [mouse] keratinocyte growth in culture was studied by both transmission and scanning electron microscopy. Under standard culture conditions (1.2-1.8 mM Ca), cells were connected by desmosomes and stratified to 4-6 cell layers. Many aspects of in vitro epidermal maturation were analogous to the in vivo process, with formation of keratohyalin granules, loss of nuclei, formation of cornified envelopes and shedding of cornified cells containing keratin filaments. When the medium Ca concentration was lowered to 0.02-0.1 M, the pattern of keratinocyte growth was strikingly changed. Cells grew as a monolayer with no desmosomal connections and proliferated rapidly, shedding largely non-cornified cells into the medium. Large bundles of keratin filaments were concentrated in the perinuclear cytoplasm. The elevation of extracellular Ca to 1.2 mM induced low Ca keratinocytes to stratify, keratinize and cornify in a manner analogous to that seen when plated in standard Ca medium. The earliest Ca-induced ultrastructural change was the asymmetric formation of desmosomes between adjacent cells. Desmosomal plaques with associated tonofilaments were observed 5 min after Ca addition; symmetric desmosomes were formed within 1-2 h. This system is presented as a useful model for the study of the regulation of desmosome assembly and disassembly.