Estrogen receptor subtype β2 is involved in neuromast development in zebrafish (Danio rerio) larvae
Estrogen receptor subtype β2 is involved in neuromast development in zebrafish (Danio rerio) larvae
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DOI：
10.1016/j.ydbio.2009.03.005
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发表时间：
2009-06-01
影响因子：
2.7
通讯作者：
Eggen, Rik I. L.
中科院分区：
文献类型：
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作者：
Froehlicher, Mirjam;Liedtke, Anja;Eggen, Rik I. L.
已知雌激素在哺乳动物的生殖和非生殖功能中发挥作用。雌激素及其受体参与中枢神经系统的发育（大脑发育、神经元存活和分化）以及周围神经系统的发育（感觉运动行为）。为了破译雌激素在斑马鱼感觉系统早期发育中的可能功能，我们通过使用吗啉代 (MO) 方法阻断 er beta(2) RNA 翻译，研究了雌激素受体 beta(2) (ER beta(2)) 的作用。我们通过细胞特异性标记进一步研究了侧线器官的发育，这揭示了变形体中神经丘发育的破坏。支持细胞正常发育和迁移。然而，变形人的神经丘中不存在感觉毛细胞。微阵列分析和随后的原位杂交表明 ERN 突变体中 Notch 信号通路的异常激活。我们得出的结论是，通过 ER beta(2) 的信号传导对于毛细胞发育至关重要，并且可能涉及神经丘成熟过程中细胞命运决定过程中与 Notch 信号传导途径的相互作用。 (C) 2009 Elsevier Inc. 保留所有权利。
Estrogens are known to play a role in both reproductive and non-reproductive functions in mammals. Estrogens and their receptors are involved in the development of the central nervous system (brain development, neuronal survival and differentiation) as well as in the development of the peripheral nervous system (sensory-motor behaviors). In order to decipher possible functions of estrogens in early development of the zebrafish sensory system, we investigated the role of estrogen receptor beta(2) (ER beta(2)) by using a morpholino (MO) approach blocking er beta(2) RNA translation. We further investigated the development of lateral line organs by cell-specific labeling, which revealed a disrupted development of neuromasts in morphants. The supporting cells developed and migrated normally. Sensory hair cells, however, were absent in morphants' neuromasts. Microarray analysis and subsequent in situ hybridizations indicated an aberrant activation of the Notch signaling pathway in ERN morphants. We conclude that signaling via ER beta(2) is essential for hair cell development and may involve an interaction with the Notch signaling pathway during cell fate decision in the neuromast maturation process. (C) 2009 Elsevier Inc. All rights reserved.