Proximal prostatic stem cells are programmed to regenerate a proximal-distal ductal axis
Proximal prostatic stem cells are programmed to regenerate a proximal-distal ductal axis
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DOI：
10.1634/stemcells.2005-0585
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发表时间：
2006-08-01
期刊：
影响因子：
5.2
通讯作者：
Wilson, E. Lynette
中科院分区：
文献类型：
--
作者：
Goto, Ken;Salm, Sarah N.;Wilson, E. Lynette
前列腺癌和良性前列腺肥大都可能起源于干细胞，这凸显了这些细胞特征的重要性。前列腺包含一个导管网络，每个导管由近端（与尿道相邻）、中间和远端区域组成。在这里，我们报告了两种能够再生前列腺组织的细胞在体内前列腺重建试验中存在于前列腺导管的不同区域。第一批细胞群（具有相当大的生长潜力）位于导管近端区域和尿道中，这些细胞的存活不需要雄激素的存在。第二种（生长潜力更有限）存在于剩余的导管区域，需要雄激素才能生存。此外，我们发现能够在体内再生功能性前列腺组织的原始近端前列腺细胞也被编程重建近端-远端导管轴。与在完整前列腺中的定位相似，在体内前列腺重建试验中，在前列腺导管的近端区域发现具有最高再生能力的细胞。原始近端细胞可在肾包膜下移植物中传代四代。总之，这些新发现阐明了原始前列腺细胞的特征，这些特征可能对治疗增生性前列腺疾病的治疗方法的发展产生影响。
Prostate carcinoma and benign prostatic hypertrophy may both originate in stem cells, highlighting the importance of the characterization of these cells. The prostate gland contains a network of ducts each of which consists of a proximal (adjacent to the urethra), an intermediate, and a distal region. Here, we report that two populations of cells capable of regenerating prostatic tissue in an in vivo prostate reconstitution assay are present in different regions of prostatic ducts. The first population (with considerable growth potential) resides in the proximal region of ducts and in the urethra, and the survival of these cells does not require the presence of androgens. The second population (with more limited growth potential) is found in the remaining ductal regions and requires androgen for survival. In addition, we rind that primitive proximal prostate cells that are able to regenerate functional prostatic tissue in vivo are also programmed to re-establish a proximal-distal ductal axis. Similar to their localization in the intact prostate, cells with the highest regenerative capacity are found in the proximal region of prostatic ducts formed in an in vivo prostate reconstitution assay. The primitive proximal cells can be passaged through four generations of subrenal capsule grafts. Together, these novel findings illustrate features of primitive prostate cells that may have implications for the development of therapies for treating proliferative prostatic diseases.