Differential Expression Analysis of Reference Genes in Pineapple (Ananas comosus L.) during Reproductive Development and Response to Abiotic Stress, Hormonal Stimuli
Differential Expression Analysis of Reference Genes in Pineapple (Ananas comosus L.) during Reproductive Development and Response to Abiotic Stress, Hormonal Stimuli
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菠萝 (Ananas comosus L.) 生殖发育过程中参考基因的差异表达分析以及对非生物胁迫、激素刺激的响应
DOI：
10.1007/s12042-019-09218-2
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发表时间：
2019-06-01
影响因子：
2
通讯作者：
Qin, Yuan
中科院分区：
文献类型：
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作者：
Chen, Huihuang;Hu, Bingyan;Qin, Yuan
菠萝（Ananas comosus L.），作为一种常见的热带水果，它是一种很好的模式，用于干旱生境适应、多果和景天科酸代谢（CAM）光合作用的进化分析和遗传研究。发育相关和/或胁迫响应基因水平的标准化的可靠的参考基因对于阐明植物发育的机制以及它们对各种环境的适应是重要的。在这项研究中，选择了10个候选参考基因，每个基因的表达稳定性进行了评估，在105个菠萝样品，包括不同的生殖器官（柱头，花瓣，萼片，胚珠，花药，花，和幼果），非生物胁迫（盐，干旱和寒冷），和激素（脱落酸，乙烯，茉莉酸，水杨酸）。结果表明，PP 2A和UBQ在菠萝生殖发育过程中稳定表达。PP 2A和CYC是抗非生物胁迫的稳定参考基因，而RAN和EF 1 α/PP 2A是各种激素的最佳候选基因。为了验证使用这些稳定表达的基因进行进一步实验的可行性，我们评估了不同样品中AcMYB 30的表达谱。我们的研究结果提供了重要的见解，菠萝植物的生长和发育，以及在这个重要的水果的耐胁迫基因和压力信号通路的信息。
Pineapple (Ananas comosus L.), a popular tropical fruit, is a good model for evolutionary analysis and genetic research on adaptation to drought habitats, multiple fruits, and crassulacean acid metabolism (CAM) photosynthesis. Reliable reference genes for the normalization of the levels of development-related and/or stress-responsive genes is important for elucidating the mechanisms of plant development as well as their adaptation to various environments. In this study, ten candidate reference genes were selected, and the expression stability of each gene was assessed across 105 pineapple samples, consisting of different reproductive organs (stigma, petal, sepal, ovule, anther, flower, and fruitlet), abiotic stress (salinity, drought, and cold), and hormones (abscisic acid, ethylene, jasmonic acid, and salicylic acid). Our results revealed that PP2A and UBQ were stably expressed during pineapple reproductive development. PP2A and CYC were the stable reference genes across abiotic stress, whereas RAN and EF1α/PP2A was the best candidates for various hormones. To validate the feasibility of using these stably expressed genes for further experiments, we evaluated the expression profile of AcMYB30 across different samples. Our results provide important insights on the growth and development of the pineapple plant as well as information on stress-tolerance genes and stress-signaling pathways in this important fruit.