Prevention of Ichthyophthirius multifiliis infestation in goldfish (Carassius auratus) by potassium ferrate(VI) treatment
Prevention of Ichthyophthirius multifiliis infestation in goldfish (Carassius auratus) by potassium ferrate(VI) treatment
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DOI：
10.1016/j.vetpar.2009.11.009
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发表时间：
2010-03-25
影响因子：
2.6
通讯作者：
Gong, Xiao-Ning
中科院分区：
文献类型：
--
作者：
Ling, Fei;Wang, Jian-Guo;Gong, Xiao-Ning
多丝小瓜虫是一种重要的淡水硬骨鱼病原菌，常给水产养殖业造成重大的经济损失。研究高铁酸钾（K_2Fe_2O_3）对小鼠的急性毒性。multifiliistheront和防止I.金鱼中的multifiliis感染。在96孔微量滴定板的每个孔中，将500只恐龙暴露于高铁酸钾（VI）浓度，观察4 h，以确定急性毒性。结果表明，I.结果表明，高铁酸钾（K_2O_3）浓度在4.80 mg/L以上时，对金鱼的致死率为100%，LC_（50）为1.71 mg/L。高铁酸钾对金鱼的LC_（50）值为42.51 mg/L。金鱼暴露于4000 theronts/鱼充气自来水（剂量先前显示，导致一致的侵扰），并与theronts接触30分钟后，用单剂量的高铁酸钾（VI）处理。暴露后每天记录感染水平和患病率。结果表明，高铁酸钾在4.80 mg/L以上浓度组能显著降低金鱼第3天鳍上滋养体的数量（P < 0.05），并能显著降低小瓜虫病的发病率（P
Ichthyophthirius multifiliis is an important freshwater teleost pathogen that often leads to significant economic losses to the aquaculture industry. The purpose of this study was to assess the acute toxicity of potassium ferrate(VI) to I. multifiliis theront and the concentration needed to prevent I. multifiliis infestation in goldfish, Carassius auratus. Five hundred theronts were exposed to concentrations of potassium ferrate(VI) in each well of a 96-well microtiter plate and observed for 4 h to determine the acute toxicity. Results showed that the exposure of I. multifiliis theronts to potassium ferrate(VI) at concentrations of 4.80 mg/L or more resulted in 100% mortality by 4 h; the LC50 value was estimated to be 1.71 mg/L Aqueous static renewal 96-h bioassays were carried out to determine the acute toxicity of potassium ferrate(VI) to goldfish. The LC50 value for potassium ferrate(VI) in goldfish was 42.51 mg/L. Goldfish were exposed to 4000 theronts/fish in aerated tap water (a dose previously shown to result in consistent infestation) and treated with a single dose of potassium ferrate(VI) after 30 min contact with theronts. Infection level and prevalence were recorded everyday after exposure. The results revealed that potassium ferrate(VI) at the 4.80 mg/L or more concentrations can significantly reduce not only the number of trophonts on the fin of goldfish on day 3 (P < 0.05), but also the prevalence of ichthyophthiriasis (P