A gene-coexpression network for global discovery of conserved genetic modules
A gene-coexpression network for global discovery of conserved genetic modules
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DOI：
10.1126/science.1087447
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发表时间：
2003-10-10
期刊：
影响因子：
56.9
通讯作者：
Kim, SK
中科院分区：
文献类型：
--
作者：
Stuart, JM;Segal, E;Kim, SK
为了在全球范围内阐明基因功能，我们鉴定了来自人类、果蝇、蠕虫和酵母的 3182 个 DNA 微阵列中共表达的基因对。我们发现了 22,163 个这样的共表达关系，每个关系在进化过程中都是保守的。这种保守性意味着这些基因对的共表达赋予了选择优势，因此这些基因在功能上是相关的。其中许多关系为新基因参与细胞周期、分泌和蛋白质表达等核心生物学功能提供了有力的证据。我们通过实验证实了其中一些联系所暗示的预测，并确定了几个基因的细胞增殖功能。通过将这些链接组装成基因共表达网络，我们发现了一些动物特异性的组件以及新进化模块和古代模块之间的相互关系。
To elucidate gene function on a global scale, we identified pairs of genes that are coexpressed over 3182 DNA microarrays from humans,flies, worms, and yeast. We found 22,163 such coexpression relationships, each of which has been conserved across evolution. This conservation implies that the coexpression of these gene pairs confers a selective advantage and therefore that these genes are functionally related. Many of these relationships provide strong evidence for the involvement of new genes in core biological functions such as the cell cycle, secretion, and protein expression. We experimentally confirmed the predictions implied by some of these links and identified cell proliferation functions for several genes. By assembling these links into a gene-coexpression network, we found several components that were animal-specific as well as interrelationships between newly evolved and ancient modules.