A comparative study on the effect of high cholesterol diet on the hippocampal CA1 area of adult and aged rats.
A comparative study on the effect of high cholesterol diet on the hippocampal CA1 area of adult and aged rats.
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DOI：
10.5115/acb.2014.47.2.117
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发表时间：
2014-06
影响因子：
1.1
通讯作者：
El-Mehi AE
中科院分区：
文献类型：
--
作者：
Abo El-Khair DM;El-Safti Fel-N;Nooh HZ;El-Mehi AE
痴呆症是当今最重要的问题之一。衰老与学习和记忆障碍有关。富含胆固醇的饮食已被证明对认知能力有害。本工作旨在比较高胆固醇饮食对成年和老年雄性白化大鼠海马区的影响。本研究选用成年和老年雄性大鼠各20只。按年龄随机分为均衡饲料组和高脂饲料组。成年大鼠15g/只/天，老年大鼠20g/只/天，连续8周。取每只大鼠的海马区连续冠状切片和血液样本。为了评估饮食效果，进行了临床、生化、组织学、免疫组织化学和形态计量学评估。与平衡饮食大鼠相比，高脂饮食成年大鼠海马区CA1区变性，锥体细胞明显减少，小血管变薄增厚，星形胶质细胞明显增多，尼氏颗粒含量明显减少。此外，高脂饮食老龄大鼠海马区衰老程度加重，并伴有阿尔茨海默样病理改变。总而言之，高胆固醇饮食对海马体有显著的有害影响，而衰老可能会宣布这种影响。因此，我们应该注意限制食物中的胆固醇摄入量，以保持健康的生活方式，从而成功地衰老。
Dementia is one of the most important problems nowadays. Aging is associated with learning and memory impairments. Diet rich in cholesterol has been shown to be detrimental to cognitive performance. This work was carried out to compare the effect of high cholesterol diet on the hippocampus of adult and aged male albino rats. Twenty adult and twenty aged male rats were used in this study. According to age, the rats were randomly subdivided into balanced and high cholesterol diet fed groups. The diet was 15 g/rat/day for adult rats and 20 g/rat/day for aged rats for eight weeks. Serial coronal sections of hippocampus and blood samples were taken from each rat. For diet effect evaluation, Clinical, biochemical, histological, immunohistochemical, and morphometric assessments were done. In compare to a balanced diet fed rat, examination of Cornu Ammonis 1 (CA 1) area in the hippocampus of the high cholesterol diet adult rats showed degeneration, a significant decrease of the pyramidal cells, attenuation and/or thickening of small blood vessels, apparent increase of astrocytes and apparent decrease of Nissl's granules content. Moreover, the high cholesterol diet aged rats showed aggravation of senility changes of the hippocampus together with Alzheimer like pathological changes. In conclusion, the high cholesterol diet has a significant detrimental effect on the hippocampus and aging might pronounce this effect. So, we should direct our attention to limit cholesterol intake in our food to maintain a healthy life style for a successful aging.