MORPHOLOGICAL ANALYSIS OF CONTRACTING AND QUIESCENT ADULT-RABBIT CARDIAC MYOCYTES IN LONG-TERM CULTURE
MORPHOLOGICAL ANALYSIS OF CONTRACTING AND QUIESCENT ADULT-RABBIT CARDIAC MYOCYTES IN LONG-TERM CULTURE
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DOI：
10.1002/ar.1092270303
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发表时间：
1990-07-01
影响因子：
--
通讯作者：
DECKER, RS
中科院分区：
文献类型：
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作者：
DECKER, ML;SIMPSON, DG;DECKER, RS
分离的兔心室肌细胞很容易适应原代培养。当肌细胞在培养底物上铺展和扁平时，肌原纤维装置保持“棒状”取向。在长期培养过程中，收缩活动的发展对维持肌原纤维装置的完整性至关重要。不能搏动的肌细胞表现出萎缩的形态迹象；相反，开始搏动的肌细胞没有这种肌原纤维组织紊乱的形态迹象。收缩装置的其他成分，包括横管系统和肌浆网的亚细胞组织，在搏动的肌细胞中保留了它们与肌原纤维的结构关系，而在静止的细胞中不保持结构关系。培养的成人心肌细胞是研究机械因素对成人心脏表型的组织和维持的影响的重要模型。
Isolated rabbit ventricular cardiac myocytes adapt readily to primary culture. As the myocytes spread and flatten over the culture substratum, the myofibrillar apparatus retains a "rod-like" orientation. Development of contractile activity is crucial in the maintenance of the integrity of the myofibrillar apparatus during prolonged culture. Myocytes that fail to beat display morphological indications of atrophy; conversely, myocytes that commence beating show no such morphological signs of myofibrillar disorganization. The subcellular organization of other elements of the contractile apparatus, including the transverse tubular system and the sarcoplasmic reticulum, retain their structural relationship with the myofibrils in beating myocytes but not in quiescent cells. Culture adult myocytes represent an important model to investigate the influence of mechanical factors on the organization and maintenance of the adult cardiac phenotype.