1064 nm near-infrared multichannel Raman spectroscopy of fresh human lung tissues
1064 nm near-infrared multichannel Raman spectroscopy of fresh human lung tissues
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DOI：
10.1002/jrs.1280
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发表时间：
2005-01-01
影响因子：
2.5
通讯作者：
Hamaguchi, H
中科院分区：
文献类型：
--
作者：
Min, YK;Yamamoto, T;Hamaguchi, H
在本实验室研制的近红外多通道拉曼系统上成功地记录了未经任何处理（如冷冻和/或福尔马林固定）的新鲜人肺组织的拉曼光谱。证实1064 nm激发是新鲜肺组织的拉曼研究的必要条件; 785 nm处的激发由于极高的荧光背景而未能检测到任何拉曼带。观察到的肺组织的拉曼光谱在正常和癌变状态之间做出了明确的区分。我们还成功地获得了高信噪比的拉曼光谱凝固的血液和碳物质吞噬在肺的清道夫细胞。这些结果证明了1064 nm近红外多通道拉曼光谱用于包括癌症在内的疾病的体内、非侵入性和分子水平临床诊断的可行性。版权所有（C）2005约翰威利父子有限公司。
Raman spectra of fresh human lung tissues without any treatment such as freezing and/or formalin fixation were successfully recorded on a near-infrared multichannel Raman system developed in our laboratory. It was confirmed that 1064 nm excitation was a requisite for the Raman study of fresh lung tissues; excitation at 785 nm failed to detect any Raman bands because of an extremely high fluorescence background. The observed Raman spectra of lung tissues made a clear distinction between the normal and cancerous states. We also succeeded in obtaining high signal-to-noise ratio Raman spectra of coagulated blood and carbon matter engulfed in the lung by scavenger cells. These results demonstrate the feasibility of 1064 nm near-infrared multichannel Raman spectroscopy for in vivo, non-invasive and molecular-level clinical diagnosis of diseases including cancers. Copyright (C) 2005 John Wiley Sons, Ltd.