OCT-4 TRANSCRIPTION FACTOR IS DIFFERENTIALLY EXPRESSED IN THE MOUSE EMBRYO DURING ESTABLISHMENT OF THE FIRST 2 EXTRAEMBRYONIC CELL LINEAGES INVOLVED IN IMPLANTATION
OCT-4 TRANSCRIPTION FACTOR IS DIFFERENTIALLY EXPRESSED IN THE MOUSE EMBRYO DURING ESTABLISHMENT OF THE FIRST 2 EXTRAEMBRYONIC CELL LINEAGES INVOLVED IN IMPLANTATION
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DOI：
10.1006/dbio.1994.1312
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发表时间：
1994-11-01
影响因子：
2.7
通讯作者：
SCHOLER, HR
中科院分区：
文献类型：
--
作者：
PALMIERI, SL;PETER, W;SCHOLER, HR
Oct-4是已知的最早表达的基因，编码在哺乳动物胚胎发育过程中受发育调节的转录因子。为了了解Oct-4在小鼠早期胚胎发育中的作用，我们对OCT-4蛋白表达的时空模式进行了分析。我们报告了OCT-4蛋白在培养细胞和小鼠胚胎中的存在，这是通过共聚焦显微镜的免疫组织化学方法确定的。Oct-4蛋白在胚胎干细胞和胚胎癌细胞中都存在，但在没有白血病抑制因子的情况下和在维甲酸存在的情况下，这些细胞在培养分化后表达下调。在胚胎中，这种蛋白质在未受精卵中含量很低，在受精后形成时主要定位于原核。OCT-4蛋白存在于所有卵裂期的细胞核中，但随着胚泡阶段滋养外胚层的分化，OCT-4蛋白仅在内细胞团中表达。这一阶段的蛋白质表达模式与先前报道的原位杂交结果很好地相关；然而，在原始内胚层细胞中发现了显著的不同，这些细胞已经开始分化并沿着滋养外胚层内表面迁移。与RNA定位研究表明原始内胚层细胞中只有低水平的Oct-4转录本形成直接对比的是，这些早期迁移细胞中的蛋白质表达高于内细胞。(C)1994年学术出版社。
Oct-4 is the earliest expressed gene known to encode a transcription factor which is developmentally regulated during mammalian embryogenesis. In order to understand the role of Oct-4 in early murine embryogenesis, we carried out an analysis of the temporal and spatial pattern of protein expression. We report the presence of Oct-4 protein in cultured cells and murine embryos as determined by immunohistochemistry using confocal microscopy. Oct-4 protein is present in both embryonic stem cells and embryonal carcinoma cells, but is down-regulated following differentiation of these cells by culture in the absence of leukemia inhibitory factor or in the presence of retinoic acid, respectively. In embryos, the protein is found at low levels in unfertilized eggs and is localized predominantly to the pronuclei upon their formation following fertilization. The protein is present in the nuclei in all cleavage stages, but following differentiation of the trophectoderm at the blastocyst stage, Oct-4 protein is only expressed in the inner cell mass. The pattern of protein expression to this stage correlates well with previously reported in situ hybridization results; however, a striking difference was seen in primitive endoderm cells which had begun to differentiate and migrate along the inner surface of the trophectoderm. In direct contrast to RNA localization studies which demonstrate that there are only low levels of Oct-4 transcripts in primitive endoderm cells, protein expression in these early migrating cells is higher than in the inner cell, mass. (C) 1994 Academic Press, Inc.