IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages
IL-2 and GM-CSF are regulated by DNA demethylation during activation of T cells, B cells and macrophages
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IL-2 和 GM-CSF 在 T 细胞、B 细胞和巨噬细胞激活过程中受到 DNA 去甲基化的调节
DOI：
10.1016/j.bbrc.2012.02.094
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发表时间：
2012-03-23
影响因子：
3.1
通讯作者：
Fan, Jun Y.
中科院分区：
文献类型：
--
作者：
Li, Yan;Ohms, Stephen J.;Fan, Jun Y.
已发现DNA去甲基化发生在许多活跃表达的细胞因子的启动子处，并且被认为在转录调控中起关键作用。虽然许多DNA去甲基化研究集中在T细胞活化，增殖和分化，但其他类型免疫细胞中DNA甲基化的变化研究较少。我们发现两种细胞因子（IL-2和GM-CSF）的表达对三种类型的免疫细胞（EL 4，A20和RAW 264.7细胞）的激活有不同的反应。使用McrBC和MeDIP方法，我们观察到DNA甲基化在全基因组水平和这些细胞因子基因的启动子处降低。参与DNA去甲基化途径的几种潜在酶/辅酶的表达似乎与免疫细胞活化相关。(C)2012 Elsevier Inc. All rights reserved.
DNA demethylation has been found to occur at the promoters of a number of actively expressed cytokines and is believed to play a critical role in transcriptional regulation. While many DNA demethylation studies have focused on T cell activation, proliferation and differentiation, changes in DNA methylation in other types of immune cells are less well studied. We found that the expression of two cytokines (IL-2 and GM-CSF) responded differently to activation in three types of immune cells: EL4, A20 and RAW264.7 cells. Using the McrBC and MeDIP approaches, we observed decreases in DNA methylation at a genome-wide level and at the promoters of the genes of these cytokines. The expression of several potential enzymes/co-enzymes involved in the DNA demethylation pathways seemed to be associated with immune cell activation. (C) 2012 Elsevier Inc. All rights reserved.