MC29-IMMORTALIZED CLONAL AVIAN HEART CELL-LINES CAN PARTIALLY DIFFERENTIATE INVITRO
MC29-IMMORTALIZED CLONAL AVIAN HEART CELL-LINES CAN PARTIALLY DIFFERENTIATE INVITRO
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DOI：
10.1016/0014-4827(91)90067-5
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发表时间：
1991-02-01
影响因子：
3.7
通讯作者：
DIETERLENLIEVRE, F
中科院分区：
文献类型：
--
作者：
JAFFREDO, T;CHESTIER, A;DIETERLENLIEVRE, F
我们建立了鹌鹑克隆性心肌细胞系，这些细胞来自于用含有v-伴菌基因的MC 29病毒注射在卵内的胚胎中发育的心脏横纹肌肉瘤。通过检测横纹肌细胞标志物的抗体对这些克隆进行表征。选择两个克隆进行进一步表征的基础上的肌源性标志物的分布类似于在正常的早期胚胎心肌细胞。然而，这些肌肉标记物在培养中随着时间的推移逐渐消失。通过将禽类心肌细胞与3 T3细胞在确定的合成培养基中结合，可以在培养中重新诱导心肌细胞分化。肌肉标记物，然后重新表达在所有的心肌细胞后，尽快第1天共培养。心脏细胞的增殖在同一时间继续。这是心脏克隆的特征，因为MC 29感染的鹌鹑成肌细胞和MC 29感染的鹌鹑成纤维细胞对3 T3结合表现出分裂反应，即，降低生长和增强分化。体外培养60代以上（6个月），细胞形态无明显变化。据我们所知，这是第一个克隆的胚胎禽心脏细胞系的描述。
We established quail clonal heart muscle cell lines from cardiac rhabdomyosarcomas developed in embryos injectedin ovowith the MC29 virus containing the v-myconcogene. These clones were characterized by means of antibodies detecting markers of striated muscle cells. Two clones were selected for further characterization on the basis of a distribution of myogenic markers similar to that in normal early embryonic cardiac muscle cells. However, these muscle markers progressively disappeared with time in culture. Cardiomyocytic differentiation could be reinduced in culture, by associating the avian cardiac cells with 3T3 cells in a defined synthetic medium. Muscle markers were then reexpressed in all cardiac cells as soon as Day 1 after coculture. Multiplication of cardiac cells continued at the same time. This is characteristic of cardiac clones since MC29-infected quail myoblasts and MC29-infected quail fibroblasts exhibited a split response to 3T3 association, i.e., decreased growth and enhanced differentiation. The cardiac clones were maintainedin vitrofor more than 60 generations (6 months) without morphological changes. To our knowledge, this is the first description of clonal embryonic avian heart cell lines.