Location and dynamics of an active promoter in Escherichia coli K-12.
Location and dynamics of an active promoter in Escherichia coli K-12.
复制标题
DOI：
10.1042/bj20111258
复制
发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Busby SJ
中科院分区：
文献类型：
--
作者：
Sánchez-Romero MA;Lee DJ;Sánchez-Morán E;Busby SJ
在本文中，我们报告，转录影响的位置的DNA靶在大肠杆菌K-12。将其染色体已被工程化以编码lac阻遏物-GFP（绿色荧光蛋白）融合体的菌株用作携带五个lac操纵基因位点的阵列的低拷贝数质粒的宿主。该菌株的单个细胞表现出弥散荧光信号，表明质粒分布在整个细胞质中。然而，携带克隆的组成型启动子的该质粒的衍生物靶向朝向宿主细胞极点的类核边缘的位置。我们的结论是，从克隆的启动子的转录驱动的质粒的位置和细菌细胞中的特定位置可能有利于基因表达。
In the present paper, we report that transcription affects the location of a DNA target in Escherichia coli K-12. A strain whose chromosome had been engineered to encode a lac repressor–GFP (green fluorescent protein) fusion was used as a host for a low copy number plasmid that carries an array of five lac operator sites. Individual cells of this strain exhibited a diffuse fluorescence signal, suggesting that the plasmid is distributed throughout the cell cytoplasm. However, a derivative of this plasmid carrying a cloned constitutive promoter is targeted to a location at the edge of the nucleoid towards the pole of the host cell. We conclude that transcription from the cloned promoter is driving the location of the plasmid and that specific locations in bacterial cells may favour gene expression.