Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta.
Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta.
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DOI：
10.1371/journal.pone.0125046
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Fang DD
中科院分区：
文献类型：
--
作者：
Kim HJ;Hinchliffe DJ;Triplett BA;Chen ZJ;Stelly DM;Yeater KM;Moon HS;Gilbert MK;Thyssen GN;Turley RB;Fang DD
棉花(Gossypium sp.)胚珠表皮细胞分化为纤维起始的数量是影响棉花产量和纤维品质的重要因素。尽管在确定调节纤维初始分化的分子机制方面做出了广泛的努力，但仅发现了少数负责纤维初始分化的基因。为了鉴定直接参与纤维起始过程的假定基因，我们使用了棉花胚珠培养技术，该技术通过外源植物激素的应用结合野生型和三种无纤维突变体之间的比较表达分析来控制纤维初始分化的时间。在处理后1小时内，向开花前野生型胚珠添加外源生长素和赤霉素，这些胚珠没有可见的纤维起始部分，从而增加了影响生长素、乙烯、ABA和茉莉酸信号通路的基因的表达。大多数通过体外植物激素处理差异表达的转录物也在植物中生长的野生型和无纤维突变体的胚珠中差异表达。除了先前显示与纤维起始分化相关的 MYB25-like 基因之外，其他几个差异表达基因，包括参与生长素信号传导的生长素/吲哚-3-乙酸 (AUX/IAA)、参与乙烯生物合成的 ACC 氧化酶和控制 ABA 分解代谢速率的脱落酸 (ABA) 8'-羟化酶，在开花前受到共同调控。野生型和无纤维突变体的胚珠。这些结果支持这样的假设：植物激素信号网络调节负责棉花纤维首字母在体外和植物中分化的基因的时间表达。
The number of cotton (Gossypium sp.) ovule epidermal cells differentiating into fiber initials is an important factor affecting cotton yield and fiber quality. Despite extensive efforts in determining the molecular mechanisms regulating fiber initial differentiation, only a few genes responsible for fiber initial differentiation have been discovered. To identify putative genes directly involved in the fiber initiation process, we used a cotton ovule culture technique that controls the timing of fiber initial differentiation by exogenous phytohormone application in combination with comparative expression analyses between wild type and three fiberless mutants. The addition of exogenous auxin and gibberellins to pre-anthesis wild type ovules that did not have visible fiber initials increased the expression of genes affecting auxin, ethylene, ABA and jasmonic acid signaling pathways within 1 h after treatment. Most transcripts expressed differentially by the phytohormone treatment in vitro were also differentially expressed in the ovules of wild type and fiberless mutants that were grown in planta. In addition to MYB25-like, a gene that was previously shown to be associated with the differentiation of fiber initials, several other differentially expressed genes, including auxin/indole-3-acetic acid (AUX/IAA) involved in auxin signaling, ACC oxidase involved in ethylene biosynthesis, and abscisic acid (ABA) 8'-hydroxylase an enzyme that controls the rate of ABA catabolism, were co-regulated in the pre-anthesis ovules of both wild type and fiberless mutants. These results support the hypothesis that phytohormonal signaling networks regulate the temporal expression of genes responsible for differentiation of cotton fiber initials in vitro and in planta.
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