Exogenous gamma-aminobutyric acid addition enhances porcine sperm acrosome reaction.
Exogenous gamma-aminobutyric acid addition enhances porcine sperm acrosome reaction.
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DOI：
10.1111/asj.13744
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发表时间：
2022-01
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影响因子：
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广泛使用的猪人工授精程序涉及使用液体储存的精液，因为很难控制冷冻解冻的猪精子的质量。因此，对猪精液的需求很高。控制和提高精子功能是猪高效繁殖的必要条件。我们之前曾报道过，γ-氨基丁酸(GABA)能促进小鼠精子获能和顶体反应。在本研究中，我们证实了GABAA受体在猪精子顶体中的存在。此外，我们还研究了GABA对猪精子功能的影响。我们没有检测到GABA对精子活力和精子蛋白酪氨酸磷酸化的任何显着影响。然而，GABA促进顶体反应，这种反应被选择性的GABAA受体拮抗剂抑制。GABA与GABAA受体结合，导致氯离子内流。我们发现，1 μ-M-氨基丁酸处理后，精子细胞内氯离子浓度增加。此外，顶体反应中有效的GABA浓度与猪精子密度呈正相关。这些结果表明，GABA及其受体可以作为顶体反应的调节剂。本研究首次报道了GABA对猪精子功能的影响。
The widely used porcine artificial insemination procedure involves the use of liquid‐stored semen because it is difficult to control the quality of frozen–thawed porcine sperm. Therefore, there is a high demand for porcine semen. The control and enhancement of sperm function are required for the efficient reproduction of pigs. We previously reported that gamma‐aminobutyric acid (GABA) enhanced sperm capacitation and acrosome reaction in mice. In this study, we demonstrated the presence of GABAA receptors in porcine sperm acrosome. Furthermore, we investigated the GABA effects on porcine sperm function. We did not detect any marked effect of GABA on sperm motility and tyrosine phosphorylation of sperm proteins. However, GABA promoted acrosome reaction, which was suppressed by a selective GABAA receptor antagonist. GABA binds to GABAA receptors, resulting in chloride ion influx. We found that treatment with 1 μM GABA increased the intracellular concentration of chloride ion in the sperm. In addition, the GABA concentration effective in the acrosome reaction was correlated with the porcine sperm concentration. These results indicate that GABA and its receptors can act as modulators of acrosome reaction. This study is the first to report the effects of GABA on porcine sperm function.
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影响因子：
4.1
作者：
Breitbart, H
通讯作者：
Breitbart, H
影响因子：
3.6
作者：
Chavez, Julio C.;Hernandez-Gonzalez, Enrique O.;Trevino, Claudia L.
通讯作者：
Trevino, Claudia L.
影响因子：
5.3
作者：
Kritis AA;Stamoula EG;Paniskaki KA;Vavilis TD
通讯作者：
Vavilis TD
影响因子：
16.6
作者：
Kerns K;Zigo M;Drobnis EZ;Sutovsky M;Sutovsky P
通讯作者：
Sutovsky P
DOI：
10.1080/01485010290099246
发表时间：
2002-09-01
期刊：
ARCHIVES OF ANDROLOGY
影响因子：
--
作者：
Hu, JH;He, XB;Yan, YC
通讯作者：
Yan, YC