Quantitative Imaging of Single, Unstained Viruses with Coherent X Rays
Quantitative Imaging of Single, Unstained Viruses with Coherent X Rays
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DOI：
10.1103/physrevlett.101.158101
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发表时间：
2008-10-10
影响因子：
8.6
通讯作者：
Miao, Jianwei
中科院分区：
文献类型：
--
作者：
Song, Changyong;Jiang, Huaidong;Miao, Jianwei
我们报告的记录和重建的X射线衍射图案从单一的，未染色的病毒，为第一次。通过将病毒颗粒的衍射图案与其周围的衍射图案分离，我们对单个病毒体进行了定量和高对比度成像。病毒粒子内病毒衣壳的结构被可视化。这项工作为从蛋白质机器和病毒到细胞器的广泛样本的定量X射线成像打开了大门。此外，我们的实验是直接转移到使用X射线自由电子激光，并代表了一个实验的里程碑，对大蛋白质复合物的X射线成像。
We report the recording and reconstruction of x-ray diffraction patterns from single, unstained viruses, for the first time. By separating the diffraction pattern of the virus particles from that of their surroundings, we performed quantitative and high-contrast imaging of a single virion. The structure of the viral capsid inside a virion was visualized. This work opens the door for quantitative x-ray imaging of a broad range of specimens from protein machineries and viruses to cellular organelles. Moreover, our experiment is directly transferable to the use of x-ray free electron lasers, and represents an experimental milestone towards the x-ray imaging of large protein complexes.