In vivo real-time analysis of intraperitoneal radiolabeled tumor cell movement during laparoscopy
In vivo real-time analysis of intraperitoneal radiolabeled tumor cell movement during laparoscopy
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腹腔镜检查期间腹腔内放射性标记肿瘤细胞运动的体内实时分析
DOI：
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发表时间：
1999
影响因子：
3.9
通讯作者：
B. Chatterton
中科院分区：
文献类型：
--
作者：
P. Hewett;M. Texler;D. Anderson;G. King;B. Chatterton
目得：已经开发了一种猪模型，以允许在休息时和二氧化碳吹入期间对放射性标记的肿瘤细胞在整个腹膜腔中的运动进行实时成像。方法：选用15头30 kg的白色家猪。在麻醉下，在腹腔镜视野下将1500万至2000万个放射性标记的人结肠直肠肿瘤细胞（LIM1215）引入到腹膜腔中进入骨盆。放射性标记的肿瘤细胞运动通过使用具有高分辨率准直器的25 cm直径的低能量移动的伽马照相机来检查。在四只猪中检查了在两小时内没有吹气的肿瘤细胞移动和分布。然后在四头猪中检查在两小时内用CO2吹入肿瘤细胞的运动和分布。在另外四只猪中，肿瘤细胞与血液混合并注射到腹膜腔中，没有吹入的效果。注意到吹气。在将LIM1215细胞注射到腹膜腔中后，通过操作腹腔内内容物检查另外三只猪。通过排气吹入CO2过滤肿瘤细胞。研究结果：在腹膜腔CO2吹入和不吹入两种情况下，均发现盆腔肿瘤细胞在腹膜内广泛分布。肿瘤细胞以较慢的速度分散在整个腹膜腔中，没有二氧化碳吹入。有吹气和无吹气时，肿瘤细胞向左上象限和右上象限移动的速率不同。在这种情况下，腹腔内血液和腹腔镜穿刺器与腹腔的长期接触增加了肿瘤细胞对穿刺器和穿刺部位的污染。在所有实验中，在腹腔镜器械上识别肿瘤细胞。结论：肿瘤细胞在静息状态和CO2吹入时均在腹腔内移动。肿瘤细胞分散的模式与CO2吹入不同。血液的存在和穿刺器与腹膜内容物的长期接触是穿刺器和穿刺部位肿瘤细胞污染的主要因素。
PURPOSE: A porcine model has been developed to allow the real-time imaging of radiolabeled tumor cell movement throughout the peritoneal cavity, both at rest and during carbon dioxide insufflation. METHODS: Fifteen 30-kg domestic white female pigs were used. Under anesthesia, 15 to 20 million radiolabeled human colorectal tumor cells (LIM1215) were introduced into the peritoneal cavity under laparoscopic vision into the pelvis. Radiolabeled tumor cell movement was examined by using a 25-cm-diameter, low-energy mobile gamma camera with high resolution collimator. Tumor cell movement and distribution during two hours without insufflation was examined in four pigs. Then tumor cell movement and distribution during two hours with CO2 insufflation was examined in four pigs. In a further four pigs, tumor cells were then mixed with blood and injected into the peritoneal cavity and the effect of no insufflationvs. insufflation was noted. A further three pigs were examined with manipulation of the intra-abdominal contents after injection of LIM1215 cells into the peritoneal cavity. Venting insufflating CO2 was filtered for tumor cells. RESULTS: Widespread intraperitoneal distribution of tumor cells from the pelvis was identified both with CO2 insufflation of the peritoneal cavity and without insufflation. Tumor cells dispersed throughout the peritoneal cavity at a slower rate without carbon dioxide insufflation. There was a differential rate of tumor cell movement to the left upper quadrant and right upper quadrant with insufflation and without insufflation. Blood within the peritoneal cavity and an extended contact of the laparoscopic trocars with the peritoneal cavity in this setting increased contamination of the trocars and trocar sites with tumor cells. Tumor cells were identified on laparoscopic instruments in all experiments. No evidence of aerosolization of tumor cells was found. CONCLUSION: Tumor cells move throughout the peritoneal cavity both at rest and during CO2 insufflation. The pattern of tumor cell dispersion differs with CO2 insufflation. The presence of blood and extended contact of trocars with peritoneal contents are a major factor in trocar and trocar site tumor cell contamination.
肿瘤细胞中 99mTc-MIBI 和 201Tl-氯化亚铊积累的差异：P-糖蛋白的作用。
DOI：
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发表时间：
1995
期刊：
The quarterly journal of nuclear medicine : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR)
影响因子：
--
作者：
Ballinger,JR;Sheldon,KM;Boxen,I;Erlichman,C;Ling,V
通讯作者：
Ling,V
人癌细胞系体外摄取阳离子六（2-甲氧基异丁基异腈）-technetium-99m。
影响因子：
11.2
作者：
Delmon-Moingeon,LI;Piwnica-Worms,D;VandenAbbeele,AD;Holman,BL;Davison,A;Jones,AG
通讯作者：
Jones,AG