The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish
The clock components Period2, Cryptochrome1a, and Cryptochrome2a function in establishing light-dependent behavioral rhythms and/or total activity levels in zebrafish
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DOI：
10.1038/s41598-018-37879-8
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发表时间：
2019-01-17
影响因子：
4.6
通讯作者：
Nishina, Hiroshi
中科院分区：
文献类型：
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作者：
Hirayama, Jun;Alifu, Yikelamu;Nishina, Hiroshi
生物钟产生行为节奏，以最大限度地提高生物体的生理效率。光通过同步细胞时钟诱导这些节奏的形成。在斑马鱼中，昼夜节律钟组分Period 2（zPER 2）和Cryptochrome 1a（zER 1a）是光诱导的，但它们的生理功能尚不清楚。在这里，我们研究了zPER 2和zPER 1a在调节运动活动和行为节律中的作用。zPer 2/zCry 1a双基因敲除（DKO）斑马鱼在短暂光照3 h后，表现出总运动活动和行为节律形成的缺陷。将DKO斑马鱼暴露于12小时的光改善了行为节律的形成，但没有总的活动。我们的数据表明，光诱导的生物钟调节器zero 2a支持DKO动物暴露于12小时光的节律性。单细胞成像分析显示，zPER 2，zPER 1a和zPER 2a在同步细胞时钟中起作用。此外，对DKO斑马鱼的微阵列分析显示，参与调节细胞代谢（包括ATP产生）的基因表达异常。总的来说，我们的研究结果表明，zPER 2，zPER 1a和zPER 2a有助于以光依赖的方式同步细胞时钟，从而有助于斑马鱼的行为节律形成。此外，zPER 2和zPER 1a可能通过调节细胞能量代谢来调节总的身体活动。因此，这些生物钟成分调节运动行为的节奏和数量。
The circadian clock generates behavioral rhythms to maximize an organism's physiological efficiency. Light induces the formation of these rhythms by synchronizing cellular clocks. In zebrafish, the circadian clock components Period2 (zPER2) and Cryptochrome1a (zCRY1a) are light-inducible, however their physiological functions are unclear. Here, we investigated the roles of zPER2 and zCRY1a in regulating locomotor activity and behavioral rhythms. zPer2/zCry1a double knockout (DKO) zebrafish displayed defects in total locomotor activity and in forming behavioral rhythms when briefly exposed to light for 3-h. Exposing DKO zebrafish to 12-h light improved behavioral rhythm formation, but not total activity. Our data suggest that the light-inducible circadian clock regulator zCRY2a supports rhythmicity in DKO animals exposed to 12-h light. Single cell imaging analysis revealed that zPER2, zCRY1a, and zCRY2a function in synchronizing cellular clocks. Furthermore, microarray analysis of DKO zebrafish showed aberrant expression of genes involved regulating cellular metabolism, including ATP production. Overall, our results suggest that zPER2, zCRY1a and zCRY2a help to synchronize cellular clocks in a light-dependent manner, thus contributing to behavioral rhythm formation in zebrafish. Further, zPER2 and zCRY1a regulate total physical activity, likely via regulating cellular energy metabolism. Therefore, these circadian clock components regulate the rhythmicity and amount of locomotor behavior.