Chitosan-halloysite nanotubes nanocomposite scaffolds for tissue engineering
Chitosan-halloysite nanotubes nanocomposite scaffolds for tissue engineering
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DOI：
10.1039/c3tb20084a
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发表时间：
2013-01-01
影响因子：
7
通讯作者：
Zhou, Changren
中科院分区：
文献类型：
--
作者：
Liu, Mingxian;Wu, Chongchao;Zhou, Changren
本文采用溶液混合和冷冻干燥相结合的方法制备了新型壳聚糖-高岭土纳米复合材料（HNTs）支架，并展示了其在组织工程中的潜在应用前景。通过光谱学和形貌分析证实了壳聚糖与HNTs之间存在氢键和静电吸引作用。界面相互作用导致壳聚糖层吸附在hnt表面。力学和热性能的测定表明，与纯壳聚糖支架相比，NC支架在抗压强度、抗压模量和热稳定性方面均有显著提高。但NC支架的吸水率降低，密度增加。所有支架都表现出高孔隙结构，HNTs对支架的孔隙结构和孔隙率几乎没有影响。为了评估细胞在材料上的附着和活力，我们在材料上培养NIH3T3-E1小鼠成纤维细胞。结果表明，当负载率为80%时，壳聚糖-HNTs纳米复合材料仍具有细胞相容性。这些结果表明壳聚糖- hnts纳米支架在组织工程或作为药物/基因载体方面具有很大的应用潜力。
This work developed novel chitosan-halloysite nanotubes (HNTs) nanocomposite (NC) scaffolds by combining solution-mixing and freeze-drying techniques, and aimed to show the potential application of the scaffolds in tissue-engineering. The hydrogen bonding and electrostatic attraction between chitosan and HNTs were confirmed by spectroscopy and morphology analysis. The interfacial interactions resulted in a layer of chitosan absorbed on the surfaces of HNTs. The determination of mechanical and thermal properties demonstrated that the NC scaffolds exhibited significant enhancement in compressive strength, compressive modulus, and thermal stability compared with the pure chitosan scaffold. But the NC scaffolds showed reduced water uptake and increased density by the incorporation of HNTs. All the scaffolds exhibited a highly porous structure and HNTs had nearly no effect on the pore structure and porosity of the scaffolds. In order to assess cell attachment and viability on the materials, NIH3T3-E1 mouse fibroblasts were cultured on the materials. Results showed that chitosan-HNTs nanocomposites were cytocompatible even when the loading of HNTs was 80%. All these results suggested that chitosan-HNTs NC scaffolds exhibited great potential for applications in tissue engineering or as drug/gene carriers.