pH-Sensitive Tubular Polymersomes: Formation and Applications in Cellular Delivery
pH-Sensitive Tubular Polymersomes: Formation and Applications in Cellular Delivery
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DOI：
10.1021/nn5004088
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发表时间：
2014-05-01
期刊：
影响因子：
17.1
通讯作者：
Battaglia, Giuseppe
中科院分区：
文献类型：
--
作者：
Robertson, James D.;Yealland, Guy;Battaglia, Giuseppe
优化纳米载体的形状会影响其与细胞的相互作用，并决定内化动力学。嵌段共聚物两亲物在水溶液中自组装成单分散结构，并已被广泛探索作为药物递送载体。然而，自组装嵌段共聚物的结构主要限于球形囊泡或球形和蠕虫状胶束。在这里，我们显示了管状聚合物囊泡，长圆柱形囊泡的控制形成和纯化。管状聚合物囊泡是从其他结构中纯化出来的，它们的形成是通过掺入生物相容性膜组分胆固醇和磷脂来操纵的。最后，我们表明这些管状聚合物囊泡与球形聚合物囊泡相比具有不同的细胞内化动力学，并且可以成功地在细胞内包裹和递送荧光牛血清白蛋白。
Optimizing the shape of a nanovector influences its interaction with a cell and determines the internalization kinetics. Block copolymer amphiphiles self-assemble into monodisperse structures in aqueous solutions and have been explored extensively as drug delivery vectors. However, the structure of self-assembled block copolymers has mainly been limited to spherical vesicles or spherical and worm-like micelles. Here we show the controlled formation and purification of tubular polymersomes, long cylindrical vesicles. Tubular polymersomes are purified from other structures, and their formation is manipulated by incorporating the biocompatible membrane components cholesterol and phospholipids. Finally we show that these tubular polymersomes have different cellular internalization kinetics compared with spherical polymersomes and can successfully encapsulate and deliver fluorescent bovine serum albumin protein intracellularly.