An Efficient Synthesis of Lysophosphatidylcholine Enriched with n-3 Polyunsaturated Fatty Acids by Immobilized MAS1 Lipase
An Efficient Synthesis of Lysophosphatidylcholine Enriched with n-3 Polyunsaturated Fatty Acids by Immobilized MAS1 Lipase
复制标题
固定化 MAS1 脂肪酶高效合成富含 n-3 多不饱和脂肪酸的溶血磷脂酰胆碱
DOI：
10.1021/acs.jafc.9b05177
复制
发表时间：
2020
影响因子：
6.1
通讯作者：
Yonghua Wang
中科院分区：
文献类型：
--
作者：
Xiumei Wang;Xiaoli Qin;Xiuting Li;Zexin Zhao;Bo Yang;Yonghua Wang
利用固定化MAS1脂肪酶催化n-3多不饱和脂肪酸(PUFA)与n-甘油-3-磷脂酰胆碱(GPC)在真空无溶剂体系中的酯化反应，合成了具有多种有益作用的N-3多不饱和脂肪酸(PUFA)溶血磷脂酰胆碱(LPC)。与Novozym 435相比，固定化MAS1脂肪酶更适合生产富含n-3多不饱和脂肪酸的LPC。在此优化条件下(酶载量为300U/g底物，温度为55℃，n-3PUFA/GPC摩尔比为20：1)，GPC转化率和LPC含量最高，分别为93.12%和90.77%。此外，分子对接实验还表明，主要反应产物为1-acyl-sn-glycero-3-lysophosphatidylcholine.(sn-1酰基LPC。这些结果表明，固定化的MAS1脂肪酶具有较高的磷脂酶活性，GPC的sn-1羟基位置具有明显的专一性。从GPC中合成高纯度的n-3PUFA富含LPC用于工业应用。
n-3 polyunsaturated fatty acid (PUFA)-rich lysophosphatidylcholine (LPC) with many beneficial effects was.effectively synthesized by immobilized MAS1 lipase-catalyzed esterification of n-3 PUFA with sn-glycero-3-phosphatidylcholine.(GPC) under vacuum in a solvent-free system. Immobilized MAS1 lipase was found to be a more suitable catalyst for the.production of n-3 PUFA-rich LPC when compared with Novozym 435. The maximal GPC conversion and LPC content.(93.12% and 90.77 mol %) were obtained under the optimized conditions (enzyme loading of 300 U/g substrate, temperature.of 55 °C, and n-3 PUFA/GPC molar ratio of 20:1). Moreover, it was observed that 1-acyl-sn-glycero-3-lysophosphatidylcholine.(sn-1 acyl LPC) was the main reaction product, as demonstrated by molecular docking. These results showed that immobilized.MAS1 lipase had high phospholipase activity with a predominant specificity for the sn-1 hydroxyl position of GPC to efficiently.synthesize highly pure n-3 PUFA-rich LPC from GPC for industrial application.