Experimental Tooth Movement Into New Bone Area Regenerated by Use of Bone Marrow-Derived Mesenchymal Stem Cells
Experimental Tooth Movement Into New Bone Area Regenerated by Use of Bone Marrow-Derived Mesenchymal Stem Cells
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DOI：
10.1597/12-232
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发表时间：
2015-07-01
期刊：
影响因子：
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通讯作者：
Tanne, Kazuo
中科院分区：
文献类型：
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作者：
Tanimoto, Kotaro;Sumi, Keisuke;Tanne, Kazuo
目的：本研究的目的是研究实验牙齿移动到再生骨在牙槽嵴裂与间充质干细胞和颗粒状碳酸羟基磷灰石scaffold.Design：人工骨缺损创建双边在上切牙地区的比格犬，以模拟牙槽嵴裂患者腭裂。取髂骨骨髓来源的间充质干细胞进行体外培养，与碳酸化羟基磷灰石复合移植于骨缺损区。对照侧仅移植碳酸羟基磷灰石。移植后6个月，将多托槽矫治器固定在上颌骨两侧的侧切牙和尖牙上，使用弹性链施加100 x g的正畸力。测量侧切牙与尖牙的距离，每月拍摄标准化X线片。结果：实验侧和对照侧牙移动均伴有再生骨吸收和新骨形成。虽然在6个月的实验期间，实验侧和对照侧获得的牙齿移动量没有差异，但对照侧的牙齿移动速率不同;而实验侧的速率是一致的。根吸收的牙齿上观察到的控制侧在一只dog.Conclusion：这表明，间充质/碳酸羟基磷灰石移植治疗有很大的潜力，作为一种新的治疗模式，骨再生腭裂患者。
Objective: The aim of this study was to examine experimental tooth movement into regenerated bone in alveolar cleft with mesenchymal stem cells and a granulated carbonated hydroxyapatite scaffold.Design: An artificial bone defect was created bilaterally in upper incisor regions of beagle dogs to simulate alveolar clefts in patients with cleft palate. The mesenchymal stem cells derived from the iliac bone marrow were cultured and transplanted with carbonated hydroxyapatite into the bone defect area. Carbonated hydroxyapatite alone was transplanted on the control side. Six months after the transplantation, multi-bracket appliances were attached to the lateral incisors and canines on both sides of the maxilla to exert an orthodontic force of 100 x g using an elastic chain. The distance between lateral incisor and canine was measured, and standardized x-ray images were taken every month. The tissue after tooth movement was evaluated by histological observation.Results: The experimental tooth movement, accompanied by resorption of regenerated bone and new bone formation, was achieved on the experimental and control sides. Although there was no difference in the amount of tooth movement obtained on the experimental and control sides during the 6-month experimental period, the rate of tooth movement varied on the control side; whereas, the rate was consistent on the experimental side. Root resorption of the tooth was observed on the control side in one dog.Conclusion: It is suggested that mesenchymal/carbonated hydroxyapatite transplantation therapy has great potential as a new treatment modality for bone regeneration in patients with cleft palate.