A new form of guanylyl cyclase is preferentially expressed in rat kidney.
A new form of guanylyl cyclase is preferentially expressed in rat kidney.
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DOI：
10.1021/bi00501a002
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发表时间：
1990-12
期刊：
影响因子：
2.9
通讯作者：
Peter S. T. Yuen;L. Potter;D. Garbers
中科院分区：
文献类型：
--
作者：
Peter S. T. Yuen;L. Potter;D. Garbers
基于可溶性和质膜形式的鸟苷酸环化酶的催化结构域的保守氨基酸序列，我们已经使用聚合酶链反应来鉴定主要在肾脏中表达的鸟苷酸环化酶的新形式。这种新形式（GC-S β 2）的cDNA编码一种76.3-kDa的蛋白质，其最接近于肺可溶性鸟苷酸环化酶的70-kDa亚基（GC-S β 1）。GC-S β 1的mRNA优先在肺和脑中表达，而GC-S β 2 mRNA在肾和肝中更丰富。一个86个氨基酸的羧基末端区域延伸超过GC-S β 1的C末端，并含有异戊二烯化/羧甲基化的共有序列（-C-V-V-L）。这是第一次证明鸟苷酸环化酶的异二聚体形式之间的异质性，并建议差异调节。
On the basis of the conserved amino acid sequences of the catalytic domain of both soluble and plasma membrane forms of guanylyl cyclase, we have used the polymerase chain reaction to identify a new form of guanylyl cyclase that is expressed principally in kidney. The cDNA for this new form (GC-S beta 2) codes for a 76.3-kDa protein, which most closely resembles a 70-kDa subunit (GC-S beta 1) of the lung soluble guanylyl cyclase. The mRNA for GC-S beta 1 is preferentially expressed in lung and brain, whereas GC-S beta 2 mRNA is more abundant in kidney and liver. An 86 amino acid carboxyl-terminal region extends beyond the C-terminus of GC-S beta 1 and contains a consensus sequence (-C-V-V-L) for isoprenylation/carboxymethylation. This is the first demonstration of heterogeneity among the heterodimeric forms of guanylyl cyclase and suggests differential regulation.