Hand-held Raman spectroscopy as a pre-screening tool for archaeological bone
Hand-held Raman spectroscopy as a pre-screening tool for archaeological bone
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手持式拉曼光谱作为考古骨骼的预筛选工具
DOI：
10.1016/j.jas.2015.03.027
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发表时间：
2015
影响因子：
2.8
通讯作者：
M. Colvard
中科院分区：
文献类型：
--
作者：
W. Pestle;Victoria Brennan;R. Sierra;Erin K. Smith;B. Vesper;G. Cordell;M. Colvard
骨胶原是各种考古分析所需的基质，包括放射性碳测年，稳定同位素分析，蛋白质组学和古代DNA。然而，用于这种分析的骨取样是破坏性的过程，并且生物分子提取是时间、劳动和资本密集型的过程。因此，出于考古和法医目的，在现场（或在偏远的博物馆、储存库或其他部署和严峻的环境中）对骨进行潜在胶原蛋白水平的预筛选的能力是非常期望的。建立在以前的评估手持光谱工具和几个最近的台式拉曼研究，并使用一个强大的选择，充分表征的古骨样品，它在这里证明，快速（30秒），非破坏性的评估，通过1064 nm拉曼光谱可以提供一个现场部署的手段，通过它来量化骨胶原蛋白含量。具体而言，发现1450 cm-1至960 cm-1的峰高比可以提供骨胶原含量的定量估计，误差为±2.8 wt%，并且那些比值大于0.1的样品均适用于分析。因此，手持拉曼光谱技术已经发展到考古学家和法医科学家现场部署的程度，这将是合理和值得的。
Bone collagen is the required substrate for a variety of archaeometric analyses, including radiocarbon dating, stable isotope analysis, proteomics, and ancient DNA. Sampling of bone for such analyses is, however, a destructive process, and biomolecular extraction is a time-, labor-, and capital-intensive process. As such, the ability to pre-screen bone for potential collagen level in the field (or in remote museums, storage repositories, or other deployed and austere environments) for archaeological and forensic purposes is highly desirable. Building on previous assessments of hand-held spectroscopic tools and several recent bench top Raman studies, and using a robust selection of well-characterized ancient bone samples, it is demonstrated here that rapid (30 s), non-destructive assessment by means of 1064 nm Raman spectroscopy can provide a field-deployable means by which to quantify bone collagen content. Specifically, it was found that the 1450 cm−1to 960 cm−1peak height ratio can provide quantitative estimates of bone collagen content with an error of ±2.8 wt%, and those samples with ratios of greater than 0.1 are uniformly suitable for analysis. Hand-held Raman spectroscopic technology has therefore evolved to the point where field deployment by archaeologists and forensic scientists would be both justified and worthwhile.