Structure, conformation in aqueous solution and antimicrobial activity of ulvan extracted from green seaweed Ulva reticulata
Structure, conformation in aqueous solution and antimicrobial activity of ulvan extracted from green seaweed Ulva reticulata
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DOI：
10.1080/14786419.2017.1408098
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发表时间：
2018-10
影响因子：
2.2
通讯作者：
T. T. Tran-T.;Hai Bang Truong;N. Y. V. Tran;Thi Minh Thu Quach;T. N. Nguyen;Minh Ly Bui;Y. Yuguchi;T. Thanh
中科院分区：
文献类型：
--
作者：
T. T. Tran-T.;Hai Bang Truong;N. Y. V. Tran;Thi Minh Thu Quach;T. N. Nguyen;Minh Ly Bui;Y. Yuguchi;T. Thanh
摘要采用红外光谱（IR）、核磁共振（NMR）、sec - mall和SAXS等方法，对越南芽庄海绿海藻（Ulva reticulata）水提工艺获得的一种抗菌活性物质进行结构分析和抑菌活性研究。该产品由鼠李糖、半乳糖、木糖、甘露糖和葡萄糖（摩尔比Rha: Gal: Xyl: Man: Glu = 1:0.12:0.1:0.06:0.03）、醛酸（22.5%）和硫酸盐（17.6%）组成。化学结构测定表明，该化合物主要由双糖[→4]β-D-GlcA(1→4)α-L-Rha3S-（1→）组成。SAXS结果表明，所研究的ulvan具有棒状粗大链构象。对封闭肠杆菌和大肠杆菌的抑菌带直径分别为20 mm和18 mm。
Abstract The aim of this study is to elucidate the structure and investigate the antimicrobial activity of an ulvan obtained by water extraction from green seaweed Ulva reticulata collected at Nha Trang sea of Vietnam by using IR, NMR, SEC-MALLS and SAXS methods. The ulvan is composed of rhamnose, galactose, xylose, manose and glucose (mole ratio Rha: Gal: Xyl: Man: Glu = 1:0.12:0.1:0.06:0.03), uronic acid (22.5%) and sulphate groups (17.6%). Chemically structural determination showed that the ulvan mainly composed of disaccharide [→4)β-D-GlcA(1→4)α-L-Rha3S-(1→]. The results from SAXS indicated that ulvan under study has a rod-like bulky chain conformation. Ulvan from U. reticulata showed high antimicrobial activity, with inhibition zone diameter of 20 mm against Enterobacter cloace and 18 mm against Escherichia coli.