Expression and function of a groEL paralog in the thermophilic cyanobacterium Thermosynechococcus elongatus under heat and cold stress
Expression and function of a groEL paralog in the thermophilic cyanobacterium Thermosynechococcus elongatus under heat and cold stress
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DOI：
10.1016/j.febslet.2008.08.034
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发表时间：
2008-10
期刊：
影响因子：
3.5
通讯作者：
Shinichiro Sato;M. Ikeuchi;Hitoshi Nakamoto
中科院分区：
文献类型：
--
作者：
Shinichiro Sato;M. Ikeuchi;Hitoshi Nakamoto
蓝藻基因组通常包含两个GroEL基因，称为groEL1和groEL2。本研究的目的是阐明groEL2在嗜热蓝藻嗜热蓝藻适应高温环境中的作用。两个GroEL基因都被发现是热诱导的，而只有groEL2基因受到很大的冷诱导。引物延伸和凝胶迁移率分析表明，groEL2的转录调控与groESL1不同。在50°C的正常生长条件下，groEL2基因是不必要的，因为groEL2干扰物是有效的。该突变株对高温和低温高度敏感。
Cyanobacterial genomes generally contain two groEL genes, referred to as groEL1 and groEL2. The purpose of this study is to elucidate a role of groEL2 in the adaptation of the thermophilic cyanobacterium Thermosynechococcuselongatus to hot environments. Both groEL genes were found to be heat-induced, while only groEL2 was greatly cold-induced. Primer extension and gel mobility shift analyses indicated that transcriptional regulation of groEL2 is different from that of groESL1. The groEL2 gene was dispensable under normal growth conditions at 50°C as a groEL2 disruptant was viable. This groEL2 mutant was highly sensitive to both high and low temperatures.