The carboxy terminus of the precursor to vasopressin and neurophysin: immunocytochemistry in rat brain.
The carboxy terminus of the precursor to vasopressin and neurophysin: immunocytochemistry in rat brain.
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加压素和神经素前体的羧基末端：大鼠脑中的免疫细胞化学。
DOI：
10.1126/science.6125034
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Chrétien,M
中科院分区：
文献类型：
--
作者：
Watson,SJ;Seidah,NG;Chrétien,M
先前已鉴定出氨基酸序列的垂体糖肽现已被认为是精氨酸加压素及其相关神经生理素前体的最终部分。使用针对该 39 个氨基酸肽和加压素的抗血清的免疫细胞化学技术来研究它们在大鼠中枢神经系统中的分布。该肽位于神经分泌大细胞核的加压素合成细胞中。阳性染色的纤维从大细胞核穿过正中隆起投射到垂体后叶。对纯合布拉特尔伯勒大鼠的研究也表明，该大鼠缺乏加压素及其相关神经素的产生，也表明该肽不存在免疫反应性。这些免疫细胞化学数据强烈表明该肽是用加压素合成的。
A pituitary glycopeptide whose amino acid sequence was previously identified has now been recognized as the final portion of the precursor to arginine vasopressin and its associated neurophysin. Immunocytochemical techniques with antiserums against this 39 amino acid peptide and vasopressin were used to study their distribution in the rat central nervous system. The peptide is located in vasopressin-synthesizing cells in the neurosecretory magnocellular nuclei. Positively stained fibers project from the magnocellular nuclei through the median eminence to the posterior pituitary. Studies of the homozygous Brattleboro rat, which is known to be deficient in the production of vasopressin and its related neurophysin, also show the absence of immunoreactivity to this peptide. These immunocytochemical data strongly indicate that the peptide is synthesized with vasopressin.