Claudin expression in Barrett's esophagus and adenocarcinoma
Claudin expression in Barrett's esophagus and adenocarcinoma
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DOI：
10.1007/s00428-005-0045-9
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发表时间：
2005-12-01
期刊：
影响因子：
3.5
通讯作者：
Kiss, A
中科院分区：
文献类型：
--
作者：
Gyorffy, H;Holczbauer, A;Kiss, A
克劳丁(Claudins，CLDN)是细胞黏附、极性和控制细胞旁溶质运输的关键分子。几项研究表明，克拉丁模式的变化在癌症的发展中起到了作用。本研究旨在检测CLDN 1、2、3、4和7在Barrett‘s食道(BE)和腺癌(ACC)中的表达模式，并与凹上皮(FOV)、正常鳞状上皮(SQ)和鳞癌(SQCC)进行比较。对125例经手术或内窥镜切除、石蜡包埋的病例进行免疫组织化学研究，并进行统计学分析。从30例石蜡包埋标本中分离BE、ACC和FOV，用于进一步的mRNA表达分析。CLDN7在所有上皮性肿瘤和癌组织中占主导地位，但在正常组织和病变组织中的表达没有差异。与SQ组相比，SQCC组CldN-1表达显著增加。与FOV相比，BE和ACC的CLDN3和CLDN4均显著升高。与BE相比，ACCs中CLDN2的表达显著增加。这是首次报道了BE和ACC与FOV和SQ在claudin表达模式上的异同。我们的数据证明了BE和ACC之间在CLDN模式上的密切联系，进一步证明BE是食道ACC之前的一种改变。
Claudins (CLDNs) are key molecules in cell adhesion, polarity, and control of paracellular solute transport. Several studies suggested that changes in claudin pattern have a role in cancer development. This study aimed to detect alterations in CLDN 1, 2, 3, 4, and 7 expression patterns in Barrett's esophagus (BE) and adenocarcinoma (ACC) compared with that in foveolar epithelium (FOV), normal squamous epithelium (SQ), and squamous cell carcinoma (SQCC). One hundred twenty five surgically or endoscopically removed, paraffin-embedded cases were studied by immunohistochemistry and analyzed statistically. BE, ACC, and FOV were dissected from 30 paraffin-embedded samples for further mRNA expression analysis. CLDN 7 was the dominating type in all epithelia and carcinomas, but its expression did not differ in normal and altered tissues. CLDN 1 expression was significantly increased in SQCC compared with that in SQ. CLDNs 3 and 4 were significantly elevated both in BE and ACC compared with that in FOV. CLDN 2 expression increased significantly in ACCs compared with that in BE. This is the first report proving similarities and differences regarding claudin expression pattern in BE and ACC compared with that in FOV and SQ. Our data prove a close link in CLDN pattern between BE and ACC, adding further evidence that BE is an alteration preceding esophageal ACC.