Optically teasing apart neural swelling and depolarization
Optically teasing apart neural swelling and depolarization
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DOI：
10.1016/j.neuroscience.2006.12.068
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发表时间：
2007-03-30
期刊：
影响因子：
3.3
通讯作者：
Rector, D. M.
中科院分区：
文献类型：
--
作者：
Foust, A. J.;Rector, D. M.
我们测量了双折射，90度散射光，和电压敏感染料的变化，从龙虾步行腿神经。关键化学试剂的系统应用揭示了快速光信号的独立细胞机制。每种药物都表现出混合效应，有些对细胞溶胀和折射率有更大的影响，有些改变膜电位。生物膜电位的变化与电压敏感染料信号密切相关，并受到那些改变膜电位的试剂的干扰。来自90度散射的光的信号证实了以下假设：大角度散射信号是由间隙空间的变化引起的，并且受到改变细胞肿胀和折射率的那些试剂的干扰。我们的结论是，可以利用多种细胞机制来测量快速光学信号。由于双折射产生比散射大得多的变化，偏振光的使用可能会导致改善成像神经活动与高时间分辨率，特别是因为双折射的变化密切对应于膜电位。(c)2007年IBRO。由爱思唯尔有限公司出版。保留所有权利。
We measured birefringence, 90 degree scattered light, and voltage sensitive dye changes from lobster walking leg nerves. Systematic application of key chemical agents revealed separate cellular mechanisms underlying fast optical signals. Each agent exhibited mixed effects, some having a greater effect on cellular swelling and refractive index, and some altering membrane potential. Birefringence changes were tightly correlated with voltage sensitive dye signals and were perturbed by those agents that altered membrane potential. Signals from light scattered at 90 degrees corroborated the hypothesis that large angle scattering signals arise from changes in the interstitial spaces and were perturbed by those agents that altered cellular swelling and refractive index. We conclude that multiple cellular mechanisms can be exploited to measure rapid optical signals. Since birefringence produces much larger changes than scattering, the use of polarized light might lead to improvements in imaging neural activity with high temporal resolution, especially since birefringence changes corresponded closely to membrane potential. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.