Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone.
Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone.
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DOI：
10.1016/j.actbio.2014.06.036
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发表时间：
2014-10
影响因子：
9.7
通讯作者：
M. Wiens;Tarek A. Elkhooly;H. Schröder;T. Mohamed;W. Müller
中科院分区：
文献类型：
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作者：
M. Wiens;Tarek A. Elkhooly;H. Schröder;T. Mohamed;W. Müller
在过去已经进行了几次尝试来制造显示聚酯聚己内酯（PCL）和多糖壳聚糖（CHS）的互补性质的杂化材料，以用于骨再生和组织工程领域。然而，这种复合材料本身通常没有成骨活性。在这里，我们报告的合成壳聚糖接枝聚己内酯（CHS-g-PCL）和随后的表征，包括结晶度，化学结构和热稳定性。在表面功能化的CHS-g-PCL与成骨生物二氧化硅通过表面固定化酶silicatein，蛋白质吸附，表面形态和润湿性进行了评估。最后，在表面官能化的CHS-g-PCL上培养成骨细胞SaOS-2细胞，随后分析细胞活力、矿物质沉积和碱性磷酸酶活性。这些表征揭示了一种复合材料，该复合材料将CHS-g-PCL的多功能特性与silicatein/生物二氧化硅涂层的成骨活性相结合，因此代表了传统使用的CHS/PCL复合材料用于生物医学应用的创新替代品，其中需要稳定的骨-材料界面。
Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering. However, such composites generally have no osteogenic activity per se. Here we report the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability. Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed. Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity. These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone–material interfaces are required.