Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus?
Does the combination of citrate and phytase exudation in Nicotiana tabacum promote the acquisition of endogenous soil organic phosphorus?
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DOI：
10.1007/s11104-016-2884-3
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发表时间：
2017-01
期刊：
影响因子：
4.9
通讯作者：
C. Giles;T. George;L. Brown;M. Mezeli;A. Richardson;C. Shand;R. Wendler;T. Darch;D. Menezes-Blackburn;P. Cooper;M. Stutter;D. Lumsdon;M. Blackwell;C. Wearing;Hao Zhang;P. Haygarth
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文献类型：
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作者：
C. Giles;T. George;L. Brown;M. Mezeli;A. Richardson;C. Shand;R. Wendler;T. Darch;D. Menezes-Blackburn;P. Cooper;M. Stutter;D. Lumsdon;M. Blackwell;C. Wearing;Hao Zhang;P. Haygarth
背景与目的植物对土壤内源磷的吸收可以减少农业系统对外源磷的需求。本研究探讨了柠檬酸盐和植酸酶分泌物在控制转基因烟草植物磷积累和土壤有机磷消耗方面的相互作用。烟草植物系，包括野生型、载体对照、具有柠檬酸盐转运蛋白（A. thaliana frd3）或真菌植酸酶（phyA：A.尼日尔，P.lymphocyte）和表达这两种性状的杂交植物系的特征在于柠檬酸流出和植酸酶渗出。单作和间作组合的单性状植物生长在低有效磷土壤（12周）。植物生物量，地上部磷的积累，根际土壤pH值和柠檬酸可提取的磷组分进行了测定。土地当量比和互补效应确定间作处理和多元线性回归预测地上部P积累的基础上植物分泌和土壤P depletion.ResultsCrossed植物株系柠檬酸和植酸酶共表达积累更多的地上部P比单一性状和间作植物处理。基于植酸酶不稳定土壤磷、柠檬酸流出量和植酸酶活性预测地上部磷积累量（Rsq= 0.58，P < .0001）。间作柠檬酸和植酸酶分泌的植物之间发生了正互补，最大的收益发生在植物处理与A。尼日尔phyA表达。结论我们首次表明，与烟草分泌的柠檬酸盐和植酸酶相关的性状协同作用可以与P的获得改善和土壤有机P的消耗有关。
Background and AimsPlant acquisition of endogenous forms of soil phosphorus (P) could reduce external P requirements in agricultural systems. This study investigated the interaction of citrate and phytase exudation in controlling the accumulation of P and depletion of soil organic P by transgenicNicotiana tabacumplants.MethodsN. tabacumplant lines including wild-type, vector controls, transgenic plants with single-trait expression of a citrate transporter (A. thaliana frd3) or fungal phytases (phyA:A. niger,P. lycii) and crossed plant lines expressing both traits, were characterized for citrate efflux and phytase exudation. Monocultures and intercropped combinations of single-trait plants were grown in a low available P soil (12 weeks). Plant biomass, shoot P accumulation, rhizosphere soil pH and citrate-extractable-P fractions were determined. Land Equivalent Ratio and complementarity effect was determined in intercropped treatments and multiple-linear-regression was used to predict shoot P accumulation based on plant exudation and soil P depletion.ResultsCrossed plant lines with co-expression of citrate and phytase accumulated more shoot P than single-trait and intercropped plant treatments. Shoot P accumulation was predicted based on phytase-labile soil P, citrate efflux, and phytase activity (Rsq=0.58,P< .0001). Positive complementarity occurred between intercropped citrate- and phytase-exuding plants, with the greatest gains in shoot P occurring in plant treatments withA. niger phyAexpression.ConclusionsWe show for the first time that trait synergism associated with the exudation of citrate and phytase by tobacco can be linked to the improved acquisition of P and the depletion of soil organic P.