The chain of command in Pseudomonas quorum sensing
The chain of command in Pseudomonas quorum sensing
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DOI：
10.1016/s0966-842x(97)01008-1
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发表时间：
1997-04-01
影响因子：
15.9
通讯作者：
Iglewski, BH
中科院分区：
文献类型：
--
作者：
Pesci, EC;Iglewski, BH
Ti质粒在植物中的COMMENT传播导致肿瘤发生，菌毛参与毒力因子的传播，使原本良性的细菌致病。然而，许多步骤需要适当的分泌和组装的功能菌毛。如果有办法控制这种DNA转移机制，既能防止毒力基因在细菌之间的有害传播，又能帮助植物进行抗病原体的基因工程，这将是一件非常有趣的事情。毛长而灵活，由简单的亚基组成，但具有与特定分子受体选择性相互作用的能力，毛是一种有价值的工具，使微小的细菌能够增加其有效尺寸并与环境相互作用。一个类似的系统被用于许多不同的目的，这并不奇怪。这种附属物可以用来测试环境的“水”，并且可以很容易地修改（例如，通过相位变化或通过改变粘附素亚基来改变纤维的受体特异性）。未来对病原菌菌毛组装的研究，例如剖析组装伴侣和菌毛亚基9- 1之间的相互作用表面，将有助于开发能够阻断细胞器组装和功能的定向药物。了解粘附素的特异性，包括P菌毛粘附素与globosidd-'结合的化学性质，结合菌毛结构5,6以及菌毛介导的粘附与毒力因子级联表达4之间的联系，可能揭示细菌与宿主细胞之间信号传递的化学基础。也许我们对菌毛结构和功能的总体理解将受益于我们对结合菌毛如何允许细胞之间的密切交流的理解，以及关于致病性菌毛的组装和粘附机制的大量信息。
COMMENT transmission of Ti plasmids into plants results in tumorigenesis, and pilus involvement in the transmission of virulence factors allows otherwise benign bacteria to cause disease. However, many steps are required for the proper secretion and assembly of a functional pilus. It will be very interesting to discover if there are ways of taming this mechanism of DNA transfer, both to prevent the harmful spread of virulence genes among bacteria and to aid the genetic engineering of plants for pathogen resistance. Long and flexible, composed of simple subunits, but with the ability to discriminately interact with specific molecular receptors, pili are a valuable tool that enables the tiny bacterium to increase its effective size and interact with its environment. The fact that a similar system is used for so many different purposes is not surprising. This appendage can be used to'test the waters' of the environment and can be easily modified (for example, by phase variation or by changing the adhesin subunit to alter the receptor specificity of the fiber). Future research on pilus assembly in pathogenic bacteria, for example to dissect the interactive surfaces between the assembly chaperone and pilus subunits9-t l, will allow development of directed agents that can block the assembly and function of the organelle. Understanding adhesin specificity, including the chemistry of P pilus adhesin binding to globosidd-', combined with pilus structure 5, 6 and the link between pilus-mediated adhesion and the cascade of expression of virulence factors 4, may reveal the chemical basis of signal transfer between bacteria and host cells. Perhaps our general understanding of pilus structure and function would profit from combining our understanding of how conjugative pill allow intimate communication between cells with the burgeoning amount of information regarding the assembly and adhesion mechanisms of pathogenic pili.