Nematicidal fluorescent pseudomonads for the in vitro and in vivo suppression of root knot (Meloidogyne incognita) of Capsicum annuum L.
Nematicidal fluorescent pseudomonads for the in vitro and in vivo suppression of root knot (Meloidogyne incognita) of Capsicum annuum L.
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DOI：
10.1002/ps.3276
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发表时间：
2012-08
影响因子：
4.1
通讯作者：
Verinder Wahla;D. K. Maheshwari;V. Bajpai
中科院分区：
文献类型：
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作者：
Verinder Wahla;D. K. Maheshwari;V. Bajpai
植物根际促生菌（PGPR），特别是假单胞菌属的荧光菌群，作为促进土壤和种子微生物的生态友好生物控制的化学品的最佳替代物，已经受到相当大的关注。从辣椒根际分离的63株铜绿假单胞菌（Pseudomonasaeruginosa）VP 1和VP 2中筛选出2株具有促生作用的菌株，经鉴定可作为辣椒根结线虫（Meloidogyne incognita）生物防治的候选菌株。结果两株菌的体外杀线虫活性和体内杀线虫活性均较好。无名氏铜绿假单胞菌VP 2对M.无名氏用两种菌株VP 1和VP 2进行种子细菌化能够处理根结M。incognita on chili（C. annuum）在盆栽试验研究中。与对照相比，根长、地上部长、地上部鲜重、干重增加，根瘤指数降低。在总体性能方面，VP 2比VP 1有效29.5%，比对照（非细菌化）有效约30%。结论铜绿假单胞菌VP 1和VP 2的应用控制了M. C. incognita;因此，它们可作为有效的植物生长促进剂和生防剂，用于提高黄瓜的健康产量。annuum，能促进植物生长，减少线虫（M.无名氏）人口。
BACKGROUND Considerable attention has been paid to plant-growth-promoting rhizobacteria (PGPR), especially the fluorescent group of Pseudomonas species, as the best alternatives to chemicals for facilitating ecofriendly biological control of soil- and seedborne microorganisms. On the basis of their novel plant-growth-promoting attributes, two rhizobacteria Pseudomonas aeruginosa VP1 and VP2 selected out of over 63 isolates from the rhizosphere of chilli (Capsicum annuum) were identified as potential candidates for biocontrol of the root-knot nematode Meloidogyne incognita on chilli. RESULTS The nematicidal activity of both strains was evaluated in vitro and in vivo for their efficacy against M. incognita. P. aeruginosa VP2 exhibited strong nematicidal activity in comparison with VP1, based on the in vitro killing of the second-stage juveniles (J2) of M. incognita. Seed bacterisation with both strains VP1 and VP2 was able to manage root-knot M. incognita on chilli (C. annuum) in a pot trial study. Increase in root and shoot length and in fresh and dry weight of root and shoot and reduction in the root-knot index over the control were attained. In overall performance, VP2 was 29.5% more effective than VP1, and about 30% more effective than the control (non-bacterised). CONCLUSION The application of P. aeruginosa VP1 and P. aeruginosa VP2 controls the development of M. incognita in C. annuum, and hence they are recommended as efficient plant growth promotors and biocontrolling agents for raising healthy crop of C. annuum that can promote the growth of plants and reduce the nematode (M. incognita) population.