Stimulation of Saccharomyces cerevisiae Cultures by Pulsed Electric Fields
Stimulation of Saccharomyces cerevisiae Cultures by Pulsed Electric Fields
复制标题
DOI：
10.1007/s11947-014-1336-4
复制
发表时间：
2014-11-01
影响因子：
5.6
通讯作者：
Vorobiev, Eugene
中科院分区：
文献类型：
--
作者：
Mattar, Jessy R.;Turk, Mohammad F.;Vorobiev, Eugene
研究了电场强度E=20-2,000 V·cm ~（-1）、有效脉冲电场处理时间t（PEF）=10 ~（-5）~（-1）s的脉冲电场（PEF）对酿酒酵母细胞水悬浮液的刺激效果。在相对高的电场强度（E> 1,000 V cm（-1））和中等的PEF处理次数（t（PEF）> 100 μ s）下，观察到从酵母中提取离子组分，这可能与细胞膜的电穿孔有关。培养皿计数结果表明，殖民地大小与发酵时间t（f）和能耗W有关。区分了“对数”和“饱和”类型的电刺激。在“对数”电刺激（10（-7）J mL（-1）< W< 10（-1）J mL（-1））下，酵母细胞的存活率随着功耗的增加而增加，并且随着发酵时间的延长（t（f）=24 h）而增加。然而，在“饱和”电刺激（10（-1）J mL（-1）< W< 10（1）J mL（-1））下，t（f）=1 h的酵母细胞活力明显高于t（f）=24 h。初步探讨了预发酵时间和PEF方案对菌体生物活性和营养物质消耗的影响。
The effects of stimulation of Saccharomyces cerevisiae cells in an aqueous suspension by pulsed electric field (PEF) with electric field strength E=20-2,000 V cm(-1) and effective PEF treatment time t(PEF)=10(-5)-1 s were investigated. At relatively high electric field strengths (E> 1,000 V cm(-1)) and moderate times of PEF treatment (t(PEF)> 100 mu s), the extraction of ionic components from yeast was observed, which can be related to electroporation of cell membranes. Petri dishes counting revealed dependency of the colony sizes on the time of preliminary fermentation t(f) and power consumption W. The "logarithmic" and "saturated" types of electrostimulation were distinguished. At "logarithmic" electrostimulation (10(-7) J mL(-1)< W< 10(-1) J mL(-1)), the viability of yeast cells increased with the increase of power consumption and was higher for longer fermentation (t(f)=24 h). However, at "saturated" electrostimulation (10(-1) J mL(-1)< W< 10(1) J mL(-1)), the viability of yeast cells was noticeably higher for t(f)=1 h than for t(f)=24 h. The impact of preliminary fermentation time and PEF protocol on biological activity of cells and consumption of nutrients was also discussed.