First Evidence of Antibodies Against Lloviu Virus in Schreiber's Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain
First Evidence of Antibodies Against Lloviu Virus in Schreiber's Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain
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DOI：
10.3390/v11040360
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发表时间：
2019-04-01
期刊：
影响因子：
4.7
通讯作者：
Negredo, Anabel
中科院分区：
文献类型：
--
作者：
Ramirez de Arellano, Eva;Sanchez-Lockhart, Mariano;Negredo, Anabel
尽管2002年在西班牙北方洞穴中的食虫性施赖伯氏弯翅蝠尸体中发现了LLOV病毒（LLOV），但其感染性和致病性仍不清楚。我们研究了LLOV的血清阳性率在潜在暴露的施赖伯氏弯翅蝙蝠（n = 60），普通蝙蝠（n = 10）作为对照，和人类（n = 22）使用免疫印迹法。我们在所有施赖伯氏弯翅蝙蝠血清池中发现了抗LLOV GP（2）的抗体，但在任何普通蝙蝠和人类血清池中均未发现。为了证实这种血清反应性，使用域可编程阵列（DPA）单独测试52种血清，DPA是用于分析丝状病毒表位的基于噬菌体展示的系统血清学技术。在19/52个测试样品中获得了针对不同LLOV蛋白的血清学特征（36.5%）。免疫显性应答大多数特异于LLOV独特表位，证实检测到的血清学应答是针对LLOV的。据我们所知，这是第一个血清学证据的LLOV暴露在活捕获的施赖伯氏弯翼蝙蝠，解离LLOV循环的原因，以前报道的死亡。
Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber's Bent-winged bats in the caves of Northern Spain in 2002, its infectivity and pathogenicity remain unclear. We examined the seroprevalence of LLOV in potentially exposed Schreiber's Bent-winged bats (n = 60), common serotine bats (n = 10) as controls, and humans (n = 22) using an immunoblot assay. We found antibodies against LLOV GP(2) in all of Schreiber's Bent-winged bats serum pools, but not in any of the common serotine bats and human pools tested. To confirm this seroreactivity, 52 serums were individually tested using Domain Programmable Arrays (DPA), a phage display based-system serology technique for profiling filovirus epitopes. A serological signature against different LLOV proteins was obtained in 19/52 samples tested (36.5%). The immunodominant response was in the majority specific to LLOV-unique epitopes, confirming that the serological response detected was to LLOV. To our knowledge, this is the first serological evidence of LLOV exposure in live captured Schreiber's Bent-winged bats, dissociating LLOV circulation as the cause of the previously reported die-offs.